Molecular and bacteriological detection of some bacterial vaginosis associate bacteria in women
العناوين الأخرى
التشخيص الجزيئي و البكتريولوجي لبعض أنواع البكتريا المرتبطة بالتهابات المهبل عند النساء
المؤلفون المشاركون
Abd, Khawlah Abd al-Amir
Kandal, Nuha Juzif
المصدر
العدد
المجلد 57، العدد 3B (30 سبتمبر/أيلول 2016)، ص ص. 1926-1936، 11ص.
الناشر
تاريخ النشر
2016-09-30
دولة النشر
العراق
عدد الصفحات
11
التخصصات الرئيسية
الموضوعات
الملخص EN
Bacterial vaginosis (BV) is one of the most common genital infections among women in the childbearing age.
Many novel, fastidious and uncultivated bacterial species are related with BV.
These are called bacterial vaginosis associated bacteria (BVAB), present in trace amount and have a significant role in the infection.
A total of 80 vaginal swabs were obtained from 80 pregnant and non-pregnant women.
Samples were collected from different hospitals in Baghdad city and Al-Kut city.
Clinically, 60 sample among 80 were gave positive results depending on Nugent score and Amsel criteria ,the Bacteriologicall test showed the percentages of gram negative bacteria (E.coli ,K.pneumoniae, P.mirabilis, Ps.aeruginosaand A.
baumanniiwere) were (38.3 ,28.3, 26.6,20,16.6,15)% respectively, while, the percentages of gram positive bacteria ( Lactobacillus spp., S.
epidermidis , S.
aureus, S.
agalactiae and S.
faecaliswere( 100, 46.6, 38.3,18.3 ,11.6)% respectively.
The results of molecular detection revealed that the specific primers amplified in 48 sample of vaginal swabs and produced a single band with a molecular weight of 210 bp for G.
vaginalisgene, and 9 samples of vaginal swabs for Leptotrichia/Sneathia gene and produced a single band with a molecular weight of 320bp,3While negative results for other bacteria such as Atopobiumvaginae and Bacterial Vagionosis Associated Bacteria 1,2,3 ( BVAB1, BVAB2 and BVAB3) .
The results of LactobacillusSpp., showed that among 60 samples of LactobacillusSpp., 28 isolates (46.66%) were L.
acidophilus and the PCR product appeared as a single DNA band with a molecular weight of 192bp,while 32 isolates (53.33%) were L.
fermentum,the PCR product appeared as single DNA band with a molecular weight of 600 bp.
Sequencing of 16s rRNA of G.vaginalisand L.fermentumwasagreed with PCR identification give similar identity in percentages (87.5 and 100)% respectively with the standard strains in NCBI web site.
While for Leptotrichia spp.
it differs and related to modified nucleic acid bacteria in comparison with NCBI web site and hence is considered as a novel BV associated bacteria in vagina.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abd, Khawlah Abd al-Amir& Kandal, Nuha Juzif. 2016. Molecular and bacteriological detection of some bacterial vaginosis associate bacteria in women. Iraqi Journal of Science،Vol. 57, no. 3B, pp.1926-1936.
https://search.emarefa.net/detail/BIM-709510
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Abd, Khawlah Abd al-Amir& Kandal, Nuha Juzif. Molecular and bacteriological detection of some bacterial vaginosis associate bacteria in women. Iraqi Journal of Science Vol. 57, no. 3B (2016), pp.1926-1936.
https://search.emarefa.net/detail/BIM-709510
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abd, Khawlah Abd al-Amir& Kandal, Nuha Juzif. Molecular and bacteriological detection of some bacterial vaginosis associate bacteria in women. Iraqi Journal of Science. 2016. Vol. 57, no. 3B, pp.1926-1936.
https://search.emarefa.net/detail/BIM-709510
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Text in English ; abstracts in English and Arabic.
رقم السجل
BIM-709510
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر