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Phenotypic and genotypic analysis of pathogenic Escherichia coli virulence genes recovered from Riyadh, Saudi Arabia
المؤلفون المشاركون
Zikri, Adil M.
Husayn, Ashjan Muhammad
Musa, Ihab Muhammad
Dawud, Turki M.
al-Mari, Khalid Salimin
al-Farraj, Abd Allah A.
Ali, Muhammad S.
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 23، العدد 6 (30 نوفمبر/تشرين الثاني 2016)، ص ص. 713-717، 5ص.
الناشر
تاريخ النشر
2016-11-30
دولة النشر
السعودية
عدد الصفحات
5
التخصصات الرئيسية
العلوم الطبية والصيدلة والعلوم الصحية
الطب البشري
الملخص EN
The current study was carried out to evaluate the phenotypic and genotypic characterization of avian pathogenic Escherichia coli recovered from Riyadh, Saudi Arabia.
During the period of 10th February–30th May 2015, 70 E.
coli strains were isolated from chicken farms located in Riyadh, Saudi Arabia.
All strains were tested phenotypically by standard microbiological techniques, serotyped and the virulence genes of such strains were detected by polymerase chain reaction (PCR).
Most of the recovered strains from chickens belonged to serotype O111: K58 25 strains (35.7 %), followed by serotype O157:H7 13 strains (18.57 %), followed by serotype O114:K90 10 strains (14.29 %), then serotype O126:K71 9 strains (12.9 %), serotype O78:K80 8 strains (11.43 %) and in lower percentage serotype O114:K90 and O119:K69 5 strains (7.14 %).
The virulence genotyping of E.
coli isolates recovered from broilers revealed the presence of the uidA gene in all the field isolates (6 serovars) examined in an incidence of 100 %, as well as the cvaC gene was also present in all field isolates (6 serovars), while the iutA gene and the iss gene were detected in 5 out of 6 field serovars in an incidence of 81.43% and 64.29 %, respectively.
Phenotypical examination of the other virulence factors revealed that 65 isolates were hemolytic (92.9 %), as well as 15 isolates (21.42 %) were positive for enterotoxin production.
Meanwhile, 21 isolates (30 %) were positive for verotoxin production, 58 isolates (82.86 %) for the invasiveness and 31 isolates (44.29 %) for Congo red binding activities of the examined serotypes.
_ 2015 The Authors.
Production and hosting by Elsevier B.V.
on behalf of King Saud University.
This is an open access article under theCCBY-NC-NDlicense (http://creativecommons.org/licenses/by-nc-nd/4.0/).
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Farraj, Abd Allah A.& Ali, Muhammad S.& Husayn, Ashjan Muhammad& Zikri, Adil M.& Dawud, Turki M.& al-Mari, Khalid Salimin…[et al.]. 2016. Phenotypic and genotypic analysis of pathogenic Escherichia coli virulence genes recovered from Riyadh, Saudi Arabia. Saudi Journal of Biological Sciences،Vol. 23, no. 6, pp.713-717.
https://search.emarefa.net/detail/BIM-717167
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Farraj, Abd Allah A.…[et al.]. Phenotypic and genotypic analysis of pathogenic Escherichia coli virulence genes recovered from Riyadh, Saudi Arabia. Saudi Journal of Biological Sciences Vol. 23, no. 6 (Nov. 2016), pp.713-717.
https://search.emarefa.net/detail/BIM-717167
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Farraj, Abd Allah A.& Ali, Muhammad S.& Husayn, Ashjan Muhammad& Zikri, Adil M.& Dawud, Turki M.& al-Mari, Khalid Salimin…[et al.]. Phenotypic and genotypic analysis of pathogenic Escherichia coli virulence genes recovered from Riyadh, Saudi Arabia. Saudi Journal of Biological Sciences. 2016. Vol. 23, no. 6, pp.713-717.
https://search.emarefa.net/detail/BIM-717167
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 716-717
رقم السجل
BIM-717167
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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