تأثير المستخلص الخام لنبات الراوند Rheum ribes في خط خلايا سرطان الحنجرة Hep-2 و وراثة الخلايا اللمفاوية البشرية النامية في الزجاج

الملخص EN

Cytotoxic effect of Rheum ribes crude extraction was studied on Human epidermoid larynx carcinoma cell line (Hep-2) within two exposure time (3,6) days.

Cytotoxic assay showed different results dependent manner.

All concentrations of extract were more toxic in 6 days compared with 3 days.

Inhibition concentration 50 (IC50) of extraction was 0.063 μg/ml after 6 days exposure time.

There was no Inhibition concentration 50 when the cells treated with extraction within 3 days.

The best Inhibitory rate of it was 27.5% when it exposed with 100 μg/ml extraction.

Opposite directions of extract action were observed.

There were stimulation in the growth of cancer cells when it treated with high dose (100 mg/ml) while , in low dose, inhibition was observed.

Proliferation Rate were 46.154 % , 25.15 % at 3 and 6 days respectively.

All lower concentrations showed deferent inhibitory rate.

There were reduction effect of Mitotic Index and Blast Index.

All concentrations showed chromosomal aberrations and sister chromatid exchanges (SCE).

Cell cycle progression (CCP) was accelerated and replicative index (RI) was arising by medium concentrations.

The opposite happened at high and lower concentration.