The effect of irradiation treatment on the non-enzymatic browning reaction in legume seeds

الملخص EN

The present study was conducted to evaluate the effects of gamma irradiation treatment, at room temperature, on the non-enzymatic browning reaction (Millerd reaction products, MRPs) generated in soybeans, broad beans and dried peas seeds at dose levels of 10, 30 and 60 kGy and their effects on the chemical constituents, soluble protein, available lysine and in vitro protein digestibility.

The formation of MRPs in the studied legumes was assayed by monitoring the formation of brown pigments (browning intensity) by spectrophotometric method.

The results revealed that the chemical composition of irradiated legumes showed non-significant differences relative to the raw one.

A dose dependent decrease in soluble proteins and available lysine in the three legumes were observed.

The non-enzymatic browning reaction was significantly increased with increasing the radiation dose, which was proved by changes in browning index tests.

At the same time, the in vitro protein digestibility was incre ased after irradiation up to 60 kGy.

Irradiation of dried peas with 60 kGy produced higher browning index than the other legumes.

A positive correlation was observed between the radiation dose and the browning index for soybeans (R2= 0.96), broad beans (R2 = 0.81) and dried peas (R2 = 0.97) which means that 96%, 81% and 97 of the variation in the incidence of non-enzymatic browning reaction in soybean, broad bean and dried peas, respectively, are due to the effect of irradiation treatments.

The present study suggests that the formation of non-enzymatic browning reaction did not impair the nutritional quality of legumes, therefore, the process of irradiation was helpful in increasing the in vitro protein digestibility of studied legumes.

These results clearly indicated that gamma irradiation processing at the studieddoses can add valuable effects to the studied legumes.