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Local preparation and evaluation of double-antibody liquid phase radioimmunoassay system for detection of human testosterone
المؤلفون المشاركون
Shafiq, H. M.
Sallam, Kh. M.
al-Shair, M. R.
Ubayd, Narin Hafiz
Muhammad, M. S.
المصدر
Arab Journal of Nuclear Sciences and Applications
العدد
المجلد 49، العدد 4 (31 أكتوبر/تشرين الأول 2016)، ص ص. 233-250، 18ص.
الناشر
الجمعية المصرية للعلوم النووية و تطبيقاتها
تاريخ النشر
2016-10-31
دولة النشر
مصر
عدد الصفحات
18
التخصصات الرئيسية
الملخص EN
Preparation, evaluation and optimization of testosterone radioimmunoassay (RIA) system using liquid phase double antibody is considered to be the main objective.
Three primary components were prepared and characterized to obtain valid and accurate system.
These components were polyclonal testosterone antibody, the 125I-testosterone tracer and set of testosterone standards.
The production of polyclonal testosterone antibody was undertaken by immunizing two groups of females white New-Zealand rabbits with testosterone-3-(O-carboxy methyl oxime): BSA as immunogen through primary immunization and five boosters.
Both R1 and R4 gave anti-serum has a high immunoreactivity.
The preparation of 125I-testosteronetracer was carried out using three different conjugates (testosterone-3-TME, testosterone-3-histamine and testosterone-3-BSA) by electrophilic substitution mechanism using chloramine-T as oxidizing agent.
Tracers were characterized in terms of radiochemical yield %, radiochemical purity %, specific activity and immunoreactivity.
A set of testosterone standards were prepared using highly purified testosterone antigen.
Optimization and validation tests of the local liquid phase RIA system were carried out.
In conclusion, the results showed that, the local testosterone RIA system is sensitive, specific and accurate with significant cost reduction in comparison with commertial kit and extended use of the method for routine investigation of variety of diseases especially hypogonadism and associated male infertility.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shafiq, H. M.& Sallam, Kh. M.& Ubayd, Narin Hafiz& Muhammad, M. S.& al-Shair, M. R.. 2016. Local preparation and evaluation of double-antibody liquid phase radioimmunoassay system for detection of human testosterone. Arab Journal of Nuclear Sciences and Applications،Vol. 49, no. 4, pp.233-250.
https://search.emarefa.net/detail/BIM-724631
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shafiq, H. M.…[et al.]. Local preparation and evaluation of double-antibody liquid phase radioimmunoassay system for detection of human testosterone. Arab Journal of Nuclear Sciences and Applications Vol. 49, no. 4 (Oct. 2016), pp.233-250.
https://search.emarefa.net/detail/BIM-724631
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shafiq, H. M.& Sallam, Kh. M.& Ubayd, Narin Hafiz& Muhammad, M. S.& al-Shair, M. R.. Local preparation and evaluation of double-antibody liquid phase radioimmunoassay system for detection of human testosterone. Arab Journal of Nuclear Sciences and Applications. 2016. Vol. 49, no. 4, pp.233-250.
https://search.emarefa.net/detail/BIM-724631
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 249-250
رقم السجل
BIM-724631
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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