Phenotypic and genotypic characterization of Clostridium perfringens associated with necrotic enteritis in broiler chickens
المؤلفون المشاركون
Amir, M. M.
Ubayd, A. H.
Dahshan, A-H. M.
Fahmi, S. S.
المصدر
Journal of Veterinary Medical Research
العدد
المجلد 23، العدد 1 (30 يونيو/حزيران 2016)، ص ص. 50-56، 7ص.
الناشر
جامعة بني سويف كلية الطب البيطري
تاريخ النشر
2016-06-30
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Necrotic enteritis; caused by Clostridium perfringens, is one of the most important enteric diseases in poultry causing severe economic losses and increased mortalities worldwide.
The current study was designed to isolate and characterize C.
perfringens from 115 broiler chicken aged 3-6 weeks were collected from 15 farms in Beni-Suef and El-Menia Governorates during the period from September 2014 to September 2015.
A total of 230 samples were aseptically collected form chicken flocks showing signs of depression, decreased feed intake, low conversion rate and diarrhea with variable daily mortalities.
Intestinal and liver samples collected from diarrheic chicken were subjected to a bacteriological examination.
It has been found that 29 (18.3%) C.
perfringens isolates was recovered from 21 chickens of which 15 (51.7%) were toxigenic.
The later typed using dermonecrotic test and PCR.
Thirteen (86.7%) isolates belonged to type A while two (13.3%) isolates belonged to type B.
Multiplex PCR was conducted with the primers mixture of alpha, beta, epsilon and iota toxins genes to determine the type of toxin among 15 of C.
perfringens isolates.
The current study revealed that 13 isolates were positive for α-toxin gene, while only two isolates were positive for alpha, beta and epsilon toxins genes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Amir, M. M.& Dahshan, A-H. M.& Ubayd, A. H.& Fahmi, S. S.. 2016. Phenotypic and genotypic characterization of Clostridium perfringens associated with necrotic enteritis in broiler chickens. Journal of Veterinary Medical Research،Vol. 23, no. 1, pp.50-56.
https://search.emarefa.net/detail/BIM-726022
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Amir, M. M.…[et al.]. Phenotypic and genotypic characterization of Clostridium perfringens associated with necrotic enteritis in broiler chickens. Journal of Veterinary Medical Research Vol. 23, no. 1 (2016), pp.50-56.
https://search.emarefa.net/detail/BIM-726022
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Amir, M. M.& Dahshan, A-H. M.& Ubayd, A. H.& Fahmi, S. S.. Phenotypic and genotypic characterization of Clostridium perfringens associated with necrotic enteritis in broiler chickens. Journal of Veterinary Medical Research. 2016. Vol. 23, no. 1, pp.50-56.
https://search.emarefa.net/detail/BIM-726022
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 54-56
رقم السجل
BIM-726022
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر