Determination of β-galactosidase activity and kinetics in the crude extract of pleurotus ostreatus hyphae in the presence and absence of heavy metals
العناوين الأخرى
قياس النشاط و المعايير الحركية لأنزيم البيتا جلاكتوسيديز في مستخلص خيوط فطر المحار بوجود و عدم وجود المعادين الثقيلة
مقدم أطروحة جامعية
مشرف أطروحة جامعية
أعضاء اللجنة
al-Tarawinah, Khalid
al-Rawashidah, Ibrahim Muhammad
Mustafa, Ahmad
الجامعة
جامعة مؤتة
الكلية
كلية العلوم
القسم الأكاديمي
قسم الأحياء
دولة الجامعة
الأردن
الدرجة العلمية
ماجستير
تاريخ الدرجة العلمية
2016
الملخص الإنجليزي
The aim of this study was to measure the activity of β-galactosidase in Pleurotus ostreatus hyphae in the presence and absence of various heavy metals.
The enzyme β-galactosidase occurs widely in the nature and has been isolated from animal and plant sources as well as microorganism.β-galactosidase (EC 3.2.1.23), known as β-Gal, is a hydrolase enzyme which helps in the hydrolysis of lactose into monosaccharides. In this study, Extraction and characterization of β-galactosidase from Pleurotus ostreatus hyphae was achieved.
Characterization studies indicated that the enzyme extracted from Pleurotus ostreatus hyphae showed highest activity at pH 3, and the optimal temperature was found to be 40°C. The activity (Vmax ) of β-galactosidase enzyme using ONPG as substrate was found to be 0.571μmol/min, and Michaelis-Menten constant ( Km) was found to be 0.307 mM.
The results showed that the β-galactosidase activity in the crude extracts of Pleurotus ostreatus hyphae was changed in the presence of different heavy metals.
The results indicated that Hg2+ and Mo2+ have an uncompetitive inhibition on the β- galactosidase activity in the extract of Pleurotus ostreatus hyphae by decreasing both Km value from 0.307 mM to 0.285 mM and 0.253 mM, and Vmax from 0.571μmol/min to 0.189 and 0.377μmol/min, respectively.
While other heavy metals such as Al3+, Cu2+, Cr3+, Zn2+ and Ni2+ showed mixed inhibition on the β-galactosidase activity in the crude extract of Pleurotus ostreatus hyphae by decreasing Vmax from 0.571μmol/min to 0.476, 0.43, 0.363, 0.425 and 0.39 μmol/min, and by increasing Km from 0.307 mM to 1.162, 0.91,1.28, 0.66 and 0.892 mM, respectively.
However, Pb2+ showed non-competitive inhibition by decreasing Vmax from 0.571μmol/min to 0.408, and showed no change on Km value.
التخصصات الرئيسية
عدد الصفحات
43
قائمة المحتويات
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Theoretical background.
Chapter Two : Literature review.
Chapter Three : Materials and methods.
Chapter Four : Results and discussion.
References.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Shulul, Tariq Harun Rashid. (2016). Determination of β-galactosidase activity and kinetics in the crude extract of pleurotus ostreatus hyphae in the presence and absence of heavy metals. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-726128
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Shulul, Tariq Harun Rashid. Determination of β-galactosidase activity and kinetics in the crude extract of pleurotus ostreatus hyphae in the presence and absence of heavy metals. (Master's theses Theses and Dissertations Master). Mutah University. (2016).
https://search.emarefa.net/detail/BIM-726128
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Shulul, Tariq Harun Rashid. (2016). Determination of β-galactosidase activity and kinetics in the crude extract of pleurotus ostreatus hyphae in the presence and absence of heavy metals. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-726128
لغة النص
الإنجليزية
نوع البيانات
رسائل جامعية
رقم السجل
BIM-726128
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر