The antibacterial potentials and synergistic effect of some plant extracts against multidrug resistant clinical pathogens

العناوين الأخرى

التأثير الضد بكتيري المحتمل و التأثير التظافري لبعض المستخلصات النباتية ضد البكتيريا السريرية الممرضة المقاومة لعدة مضادات حيوية

مقدم أطروحة جامعية

Abu Daraz, Wisam Sulayman

مشرف أطروحة جامعية

al-Bashiti, Tarik
Abu al-Khayr, Imad

الجامعة

الجامعة الإسلامية

الكلية

كلية العلوم

القسم الأكاديمي

قسم العلوم الحياتية

دولة الجامعة

فلسطين (قطاع غزة)

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2015

الملخص الإنجليزي

The present study was designed to screen in vitro antibacterial and synergistic activity of Allium sativum, Ecballium elaterium, Pelargonium graveolens, Rosmarinus officinalis, Phagnalon rupestre & Ruta-graveolens plants against multidrug resistant clinical pathogenic bacteria.

The active compounds were extracted from the dried aerial parts of plants where successively extracted with aqueous, 80% ethanol and methanol solvents by using soxhlet extractor, and Essential Oils (EOs) which extracted from the fresh aerial parts of plant by using steam distillation.

All extracts were screened for their antibacterial activity and synergistic effect in combination with known antimicrobial agents including.

Ciprofloxacin (CIP), Ampicillin (AM), Cefotaxime (CTX), Nalidixic acid (NA), Norofloxacin (NOR), Cefuroxime (CXM), Cefaclor (CF or CEC), Ofloxacin (OFX), Cefalexin (CL or CN), Tetracycline (TE), Rifampicin (RIF), Amoxyclav (AMC), Gentamycin (GMN), Penicillin (P) & Oxacillin (OX), by using the disk diffusion method.

The minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) of the plant extracts were assessed by using micro-dilution technique.

The microorganisms used for evaluation the antibacterial and synergistic activity were clinically isolates multi-drug-resistant (MDR) bacteria.

Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae & Pseudomonas aeruginosa.

Results: The results revealed that, the average diameter of inhibition zones that resulting from the effect of plant extracts against the tested bacteria ranged from 7 to 14 mm, 7.33 to 16.66 mm, 7.66 to 17 mm and 7.33 to 12.66 mm for aqueous, ethanol, methanol & EOs extracts, respectively.

The extracts showed antibacterial activity were subjected to minimum inhibitory concentration and minimum bactericidal concentrations assay; a micro-broth dilution assay was performed on 96-well plates using 2, 3, 5-Triphenyl Tetrazolium Chloride (TTC) as an indicator for bacterial growth.

The average minimum inhibitory concentrations (MICs) values ranged from 1.562 to 100 mg/ml, 1.562 to 50 mg/ml, 1.562 to 50 mg/ml & 3.125 to 100 μl/ml for aqueous, ethanol, methanol & EOs extracts, respectively.

While minimum bactericidal concentrations (MBCs) values VI ranged from 25 to > 200 mg/ml, 25 to > 200 mg/ml, 25 to 200 mg/ml & 50 to > 200 μl/ml for aqueous, ethanol, methanol & EOs extracts, respectively.

Synergistic activity of the plant extracts when combined with antibiotics had different degree of synergism against the selected microorganisms, where in case of aquatic extracts; Rosmarinus officinalis had the best synergism against S.

aureus, E.

coli & K.

pneumoniae, while the best synergism against P.

aeruginosa was observed with Ruta-graveolens.

In case of ethanolic extracts, the best synergism was observed with Rosmarinus officinalis against S.

aureus & E.

coli, and with Ruta-graveolens & Pelargonium graveolens against K.

pneumoniae & P.

aeruginosa respectively.

In addition, the best synergism was observed with methanolic extracts of Rosmarinus officinalis, Ruta-graveolens, Ecballium elaterium & Pelargonium graveolens against S.

aureus, E.

coli, K.

pneumoniae & P.

aeruginosa respectively.

While EOs of screened plants had the best synergism against S.

aureus, E.

coli and K.

pneumoniae with Pelargonium graveolens EO for all, and had the best synergism against P.

aeruginosa with Allium sativum EO.

Conclusions: The overall results of the present work provide baseline information for the possible use of the studied plant extracts and EOs in the treatment of bacterial infections involving MDR phenotypes.

In addition to these antibacterial activities, the results indicate that the possibility of concurrent use of these antimicrobial drugs and plant extracts in combination in treating infectious diseases caused by multi-drug-resistant Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae & Pseudomonas aeruginosa or at least the concomitant administration may not impair the antimicrobial activity of some of these antibiotics.

التخصصات الرئيسية

الأحياء

عدد الصفحات

156

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-727239