Comparison of matrix-assisted laser desorption ionization-time of flight mass spectrometry, vitek-2 and id32staph for species identification of clinical coagulase-negative staphylococci isolated from al Shifa hospital in Gaza Strip
العناوين الأخرى
مقارنة بين قدرة تقنيات (MS TOF-MALDI) و (2 (VITEK و(ID32STAPH) في تشخيص أجناس و أنواع المكورات العنقودية السريرية سلبية التخثر (CoNS) المعزولة من مستشفى الشفاء في قطاع غزة
المؤلف
المصدر
IUG Journal of Natural Studies
العدد
المجلد 25، العدد 1 (30 يونيو/حزيران 2017)، ص ص. 21-29، 9ص.
الناشر
الجامعة الإسلامية-غزة عمادة شؤون البحث العلمي و الدراسات العليا
تاريخ النشر
2017-06-30
دولة النشر
فلسطين (قطاع غزة)
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Staphylococcal species, notably, coagulase negative staphylococci (CoNS), are considered important causative agents of health-care associated infections (HAI) associated with catheters and implanted medical devices.
The increasing incidence of CoNS in HAI confirms the need for an accurate and simple identification method at the species level.
Recently, matrixassisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was introduced as a useful and simple method for the rapid identification of CoNS.
In this study we compared MALDI-TOF MS, VITEK-2 and ID32STAPH for phenotypic identification of CoNS at species level.
Eighty one clinical isolates of CoNS representing six species that were collected from different clinical samples were analyzed.
All CoNS isolates were tested for catalase, Pastorex™, coagulase, nuc and mecA genes.
Our results showed correct species identification by MALDI-TOF MS which was obtained in 98.8% in comparison to 96.3% and 75.3% correct identification for the VITEK-2 and ID32STAPH respectively.
Only one strain was identified by MALDI-TOF MS at the genus level.
In addition, MALDITOF MS identified staphylococci to subspecies level including Staphylococcus hominis subsp.
novobiosepticus and subsp.
hominis; S.
saprophyticus subsp.
saprophyticus.
S.
auricularis was only identified by MALDI-TOF-MS.
There were 60 (74.1%) methicillin resistant CoNS (MRCoNS) that showed mecA positive.
Using MALDI-TOF MS technique, we were unable to discriminate between MR-CoNS and MS-CoNS, because the topology of dendrogram generated from the spectra of MR-CoNS and MSCoNS strains were almost the same.
These findings confirm the value of MALDI-TOF MS as simple, accurate and rapid method for phenotypic identification of clinical CoNS isolates.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Lahham, Nahid Ali. 2017. Comparison of matrix-assisted laser desorption ionization-time of flight mass spectrometry, vitek-2 and id32staph for species identification of clinical coagulase-negative staphylococci isolated from al Shifa hospital in Gaza Strip. IUG Journal of Natural Studies،Vol. 25, no. 1, pp.21-29.
https://search.emarefa.net/detail/BIM-735586
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Lahham, Nahid Ali. Comparison of matrix-assisted laser desorption ionization-time of flight mass spectrometry, vitek-2 and id32staph for species identification of clinical coagulase-negative staphylococci isolated from al Shifa hospital in Gaza Strip. IUG Journal of Natural Studies Vol. 25, no. 1 (2017), pp.21-29.
https://search.emarefa.net/detail/BIM-735586
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Lahham, Nahid Ali. Comparison of matrix-assisted laser desorption ionization-time of flight mass spectrometry, vitek-2 and id32staph for species identification of clinical coagulase-negative staphylococci isolated from al Shifa hospital in Gaza Strip. IUG Journal of Natural Studies. 2017. Vol. 25, no. 1, pp.21-29.
https://search.emarefa.net/detail/BIM-735586
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 27-29
رقم السجل
BIM-735586
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر