Production, purification and characterization of alginase from Bacillus circulans local isolate
العناوين الأخرى
إنتاج و تنقية و توصيف أنزيم الألجنيز من بكتريا Bacillus circulans المعزولة محليا
مقدم أطروحة جامعية
مشرف أطروحة جامعية
الجامعة
جامعة بغداد
الكلية
معهد الهندسة الوراثية و التقانات الإحيائية
دولة الجامعة
العراق
الدرجة العلمية
ماجستير
تاريخ الدرجة العلمية
2015
الملخص الإنجليزي
Fourteen bacteria isolates “ Bacillus species” were taken from Ramadi and Fallujah hospital in the period between October to December 2013, those isolated were cultured on a blood agar to test their ability to hydrolytic due to formation the inhibition zone .
Six isolate were selected to be cultured in alginate yeast extract broth for testing their efficiency to produce alginase .The efficient isolate was diagnosed depending on phenotypic, microscopic and biochemical tests to be Bacillus circulans R . The optimal conditions for alginase production by Bacillus circulans R as follows; the optimum incubation period was 24 hrs, which gave enzymatic activity (106.1U/ml) .
The optimum inoculum optimum inoculum volume was 6x107 cell/g dry weight, which gave activity (111 Unit/ml).
The optimum carbon source was sodium alginate which gave activity (212.86 U/ml), while peptone was the optimum nitrogen sources with enzymatic activity of (275 U/ml ).
The optimum pH was 7.5 which gave maximum activity (211.63 U/ml) , the shaking incubator was gave a highest production of the enzyme activity (210 U/ml). The enzyme from Bacillus circulans R was purified by several steps, Including ammonium sulfate precipitation with a saturation percentage of 50-75% which was gave specific activity (2933.15U/mg) with a fold number of (3.49) and a yield of ( 58.75%).
Ionic exchange chromatography using DEAE-Sephacel column ( 3.5 x8 cm) gave specific activity (5362.33U/mg), a fold number of (6.37) and a yield of (53.11%), then gel filtration using Sepharose 6 B column (1.5x87 cm) gave purified enzyme with specific activity of (10865U/mg), a fold number of (12.91) and a yield (28.69%).
The results of alginase characterization were: · The optimum pH for alginase activity was 7.0. · The optimum pH for enzyme stability was 7.5 . · The optimum temperature for alginase activity was 35°C. · The enzyme retained its entire activity upon 1 hour incubation at temperature 25°C . · The molecular weight of the alginase as determined by gel filtration chromatography using Sepharose 6 B gel was 40738 Dalton and by SDSelectrophoresis was49000 Daltons. The gene responsible for controlling production of alginase in Bacillus circulans R was located in the chromosomal DNA.
The alginase has ability to degrade alginate coated Pseudomonas aeruginosa when treated with it after incubated for one hour
التخصصات الرئيسية
عدد الصفحات
108
قائمة المحتويات
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Introduction.
Chapter Two : Literature review.
Chapter Three : Materials and methods.
Chapter Four : Results and discussion.
Conclusions and recommendations.
References.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Halbusi, Rafal Ismail Ali. (2015). Production, purification and characterization of alginase from Bacillus circulans local isolate. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-736084
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Halbusi, Rafal Ismail Ali. Production, purification and characterization of alginase from Bacillus circulans local isolate. (Master's theses Theses and Dissertations Master). University of Baghdad. (2015).
https://search.emarefa.net/detail/BIM-736084
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Halbusi, Rafal Ismail Ali. (2015). Production, purification and characterization of alginase from Bacillus circulans local isolate. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-736084
لغة النص
الإنجليزية
نوع البيانات
رسائل جامعية
رقم السجل
BIM-736084
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر