طفرات أنظمة التكرارات الترادفية القصيرة STRs في تحاليل النسب

الملخص EN

Background& Objective: A study of the consecutive cases for STRs mutations has been performed during years from 2007 to 2010 in Laboratory for Research and Genetic Consultation at the Faculty of Medicine, which were 44 cases investigated ratios.

Materials & Methods: 44 cases examined to confirm the ratios in the laboratory.

DNA was extracted from blood samples.

The PCR products were typed using an ABI PRISM 310 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA)).

The results were analyzed using Gene Mapper ID v3.0 software (Applied Biosystems).

As was my case study to prove the existence of mutations rates and one of them associated boom in location A Locus in the HLA system where systems have been added sexual STRs on chromosome X to this case to confirm the descent.

The study was conducted statistical cases containing mutations to calculate the probability of paternity W calculates the boom or not calculated.

Results: During the study found, 2 STRs mutations discoverers using STRs Systems in investigating cases of percentages in the following systems were D3S1358, D13S317, and in both cases were from the father's side, In paternity investigation cases, 2 STRs mutations have been detected in several loci.

The final probability paternity value (after inclusion of mutations) and that the probability of paternity in cases calculates boom with the 15 systems of STRs with the addition of other systems was always >99.99%.

Conclusion: Allowing to conclude confidently the presence of a mutation.

we can expect to find paternity investigation cases with one or even two genetic incompatibilities that are non-exclusion cases