معايرة الديجوكسين بطريقة المقايسة المناعية المرتبطة بالإنزيم (ELISA)‎ في نبات الديجيتال الأرجواني Digitalis purpurea الخاضع لتأثير الملوحة

الملخص EN

Background & Objective: The plant creates raw materials through primal metabolism in order to maintain crucial lively operations.

While in the case that the plant is subjected to inhibition of growth as a result to environmental circumstances characterized by a high level of salinity, the Digitalis Purpurea used in this experiment changes its biological potential like bio-creation to form other necessary materials that take part in secondary metabolism operations, and hence affect the creation of glycosides that strengthen the heart, which Digoxin is one of.

Material& methods: Digitalis purpurea plants were planted in different assays including 4 sets, the 1st set is the witness "control", different concentrations of the NaCl solution are added to the three remaining sets, and they are respectively mM 80, 40, 20 within a time period of 20 weeks, then an operation of Assaying Digoxin by way of Enzyme Linked Immune Standardized Assaying (ELISA) was performed.

Results: Digoxin content increased in the leaves and roots of the Digitalis Purpurea plant, planted in the witness "control" assay, or in the low salinity level assays with 20mM concentration in the 23rd and 24th weeks respectively, then it decreased.

Digoxin content in plant roots in 40mM salinity reach its maximum in the 19th week, and that agrees with the ionic relation K+ /Na+ less than /1/, and at the relation value Na+ /Ca2+ higher than 0.35.

Conclusion: The slight ionic disorder leads to an increase in the accumulation of Digoxin in the root, and more in the leaves of the Digitalis Purpurea plant.