Effect of Aloe Vacillans leaves extract on CCl4-induced hepatotoxicity in rats

الملخص EN

This Study was designed to evaluate the effects of Aloe vacillans leaves juice on carbon tetrachloride CCl4- induced hepatotoxicity in rats.

Hepatotoxicity was induced in rats by intraperitoneal (i.p) injection of CCl4 (1ml/kg) of body weight every 72h during ethanolic extract of Aloe vacillans leaves were administrated at dose 250 mg/kg and 500 mg/kg of body weight pass orally (p.o) daily for 14 days.

Twenty-four hours post-CCl4 treatment, blood samples were withdrawn through retro orbital sinus.The hepatotoxicity and its prevention was assessed by serum parameters like alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein (T.P) and albumin (ALB).

In CCl4 treated rats, a significant decreasing in the relative body weight (212g) compared with normal group (231g), increasing in the liver enzymes levels ALT (254.6±6.6 IU/L), AST (322.9±4.42 IU/L) when compared with normal group (50.7±1.5 IU/L), (70.12±2.6 IU/L) respectively and decreasing the in T.P (4.5±0.17 g/dl) and albumin levels (2.65±0.12 g/dl) were shown (p<0.05) when compared with normal group (6.9±0.12 g/dl , 3.64±0.66g/dl) respectively.

Treatment with the ethanolic extract of aloe vacillans 250 mg/kg could significantly decrease in the liver enzymes levels ALT (185.9±6.2 IU/L), AST (242.5±4.2 IU/L) when compared with CCl4- treated group (254.6±6.61,32 IU/L), (322..9±4.42 IU/L) respectively, and increased in the T.P (5.1±0.09 g/dl) and albumin level (2.28±0.01 g/dl) at p<0.05 when compared with CCl4 –treated group (6.9±0.12 g/dl), (3.64±0.66 g/dl) respectively.

Treatment with the ethanolic extract of aloe vacillans 500 mg/kg could significantly decrease in the liver enzymes levels ALT (175.6±6.4 IU/L), AST( 235±12.8 IU/L) when compared with CCl4- treated group (254.6±6.61,32 IU/L), (322..9±4.42 IU/L respectively, and increased in the T.P (5.4±0.12 g/dl) and albumin level 2.86±0.10 g/dl at p<0.05 when compared with CCl4- treated group( 6.9±0.12 g/dl), (3.64±0.66 g/dl) respectively.

The body weight in the ethanolic extracts treated rats was reduced (209.39±0.27g), compared with the body weight in CCl 4- treated rats.

(212.4±2.145 g).

The data suggested that oral administration of ethanolic extract of the leaves of Aloe vacillans significantly decreases the intensity of hepatic damage induced by CCl4 in rats