Histological alterations in kidney and liver of laboratory mice following intramuscular injection of montevipera bornmuelleri (Werner, 1898) Venom
المؤلفون المشاركون
Kossaifi, Eliane
Hraoui Bloquet, Suad
Sadiq, Riyad
Fajlun, Ziyad
Accary, Claudine
Hlihil, Walid
المصدر
العدد
المجلد 18، العدد 1 (30 يونيو/حزيران 2017)، ص ص. 122-135، 14ص.
الناشر
تاريخ النشر
2017-06-30
دولة النشر
لبنان
عدد الصفحات
14
التخصصات الرئيسية
الموضوعات
الملخص EN
Histopathological changes after bites of the Montivipera bornmuelleri viper, endemic to high altitudes areas of Lebanon, have not yet been investigated.
Our study focuses on histological changes and irreversible damages in the kidney and liver of white laboratory Balb/c mice that received an intramuscular injection of lyophilized venom diluted in saline solution (NaCl 0.9%).
Different venom concentration doses ranging from 0.25 mg/kg to 15mg/kg in a total injection volume of 100μl was injected intramuscularly (IM) into 5 groups of mice.
After dissection, observations showed no macroscopically identifiable damages in any of the organs studied.
However, tissue samples from the liver and the kidney were obtained for histological studies at various time intervals following the venom injection.
The histological study was carried out using the Bouin solution (fixing bath), followed by dehydration in alcohol.
Paraffin-embedded sections were stained using hemalun-easine.
Tissues from 6 control mice, which received only an injection of saline solution, were also examined.
The common histological alterations observed microscopically in the liver and the kidney were: pycnotic nuclei, necrosis, vacuolization, cytoplasmic destruction, edema, hemorrhage and congestion of blood vessels.
Moreover, inflammatory infiltration of lymphocytic cells was observed in the prevascular regions of both organs.
The histological changes observed appeared within 1h and intensified with time.
These changes were proportional to the dose of the venom injected into the laboratory mice.
Our results on what happens in the kidney and liver of mice after injecting the venom of M.
bornmuelleri had many similarities with the effect of venom of other vipers (e.
g.
Daboia palestinea, Bitis ssp, Bothrops moujenis etc…) observed by others authors.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kossaifi, Eliane& Hraoui Bloquet, Suad& Sadiq, Riyad& Fajlun, Ziyad& Accary, Claudine& Hlihil, Walid. 2017. Histological alterations in kidney and liver of laboratory mice following intramuscular injection of montevipera bornmuelleri (Werner, 1898) Venom. Lebanese Science Journal،Vol. 18, no. 1, pp.122-135.
https://search.emarefa.net/detail/BIM-752327
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hraoui Bloquet, Suad…[et al.]. Histological alterations in kidney and liver of laboratory mice following intramuscular injection of montevipera bornmuelleri (Werner, 1898) Venom. Lebanese Science Journal Vol. 18, no. 1 (2017), pp.122-135.
https://search.emarefa.net/detail/BIM-752327
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kossaifi, Eliane& Hraoui Bloquet, Suad& Sadiq, Riyad& Fajlun, Ziyad& Accary, Claudine& Hlihil, Walid. Histological alterations in kidney and liver of laboratory mice following intramuscular injection of montevipera bornmuelleri (Werner, 1898) Venom. Lebanese Science Journal. 2017. Vol. 18, no. 1, pp.122-135.
https://search.emarefa.net/detail/BIM-752327
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 132-135
رقم السجل
BIM-752327
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر