Sidr honey inhibitory effect on virulence genes of MRSA strains from animal and human origin

الملخص EN

In order to evaluate the effect of sidr honey on the presence of meca, coa and spa genes as well as its antibacterial activities against MRSA strains.

A total of (200) clinically mastitic milk samples and (170) samples (45 wounds, 30 sputum, 25 blood, 30 aspirates, 20 urine and 20 cerebrospinal fluids) were collected from human patients at hospitals in Sharkia Governorate.

Five different sidr honey samples from {Egypt (E), Libya ( L) ,Yemen (Y), Pakistan (P) and Saudi Arabia (S) } were used in this study .

After the bacteriological examination of the collected samples, the percentage of S.aureus was 31% in mastitic cases and 52.9% in human patients, while the percentage of MRSA strains was found to be 22.58% and 44.4%, respectively.

Meca, coa and spa genes were detected by PCR in MRSA strains before and after the exposure to sidr honey.

The absence of ( meca, coa and spa) gnes in MRSA strains after exposure to sidr honey was noticed.

Briefly, sidr honey has an inhibitory effect on (mec, coa and spa) genes of MRSA.