Pathological changes inducing by S. aureus in immunodeficiency mice immunized with Soluble Culture Filtrate S. aureus antigens (SCFAgs)‎

الملخص EN

In order to determine the influence of Soluble Culture Filtrate S.aureus Antigens (SCFAgs) on S.aureus infection in Mitomicin c immunosupression mice, seventy four white mice, both sex,7-8 weeks age were divided randomly into five groups.1st group(n=16 ) was immunized with 0.4ml of S.aureus CFSAgs (concentration of protein( 4.2mg/ml) ,i/p two doses, 2 weeks intervals.

2nd group(n=16) was injected with mitomycine C ,(1mg/kg B.W) I/p three time /week for 4 weeks.

3ed group (n=16) was immunized with CFSAgs as 1st group and treated with mitomycin as 2nd group.

4th group(n=10) was inoculated with (0.4ml) I/P with1X109 CFU/ML of viable virulent.

S.aureus and was served as control positive group.

5th group (n=16) was inoculated with 0.5ml sterile normal saline.

Cellular and humoral immune response were recorded at 28-30 day post immunization, skin test and passive heam agglutination test respectively, then all animals of immunized and treatment groups were challenge with S,aureus as control positive group.

The results explained that animals treatment with MMC were died during (18) hrs post inoculation with virulent viable S.aureus with very heavy bacterial isolation, animal of control positive group were died at( 24)hrs post infection with heavy bacterial isolation The results revealed that immunization with CFSAgs elicited both humoral and cellular immune responses, the level values of both arms of immune response were lower animal treatment with MMC, Severe pathological lesions were seen in examined organs of control positive group but these lesions are more extensive in animal treatment with MMC.

The main lesions in examined organs of these animals are suppurative inflammation ,congestion ,apoptosis and necrosis..

We conclusion that MMC induce immunosuppression condition and immunization with CFSAgs can improve the immune responses in the animals that are suffering from immunosuppression.