The hr2 haplotype in patients with deep venous thrombosis in Duhok

الملخص EN

Background: Venous thrombosis is a multifactorial disorder, with a multitude of acquired and inherited risk factors implicated.

Among the less investigated controversial inherited risk factors is the HR2 haplotype.

Materials and Methods: A total of 70 patients attending the Azadi Teaching Hospital in Duhok with Color Doppler confirmed deep venous thrombosis and seventy age and sex matched healthy controls were recruited.

DNA was extracted by phenol-chloroform based method then the HR2 haplotype was screened for using a Restriction fragment Length Polymorphism-Polymerase chain reaction method.

Results: The patients had ages ranging from 12-81 (Median 45 years), with a Male: female ratio of 1.33:1.

The controls on the other hand had ages ranging from 14-78 years (Median 43 years), and a Male: Female ratio of 1.12:1.

A total of 4 patient (5.7%) and 4 controls (5.7%) were identified as carriers of the HR2 haplotype, Three of the patients and four of the controls were heterozygous for the mutation, while the remaining one patient was homozygous for it.

There was no significant difference in the frequency of the HR2 haplotype between patients and controls (p=1.0).

Furthermore, no significant association was found within the patients’ group between the HR2 haplotype and age (p=0.147), though slightly significantly higher number of females were HR2 carriers (p=0.03).

Conclusion: The current study has documented that among the patients with venous thrombosis in Duhok, the HR2 haplotype does not appear to carry a significant thrombotic risk on its own, further larger studies including concomitant screening for other thrombophilic mutations may determine whether this mutation may increase the risk associated with these factors