Immunohistochemical evaluation of the effects of nitrate in drinking water on laminin alpha-5 expression in rat renal glomeruli
المؤلفون المشاركون
Dorafshani, Mahmud Moghaddam
Nikravesh, Muhammad Rida
Jalali, Mahdi
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 18، العدد 12 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.
الناشر
تاريخ النشر
2016-12-31
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Background: Nitrate is a polyatomic ion with the molecular formula NO3 -.
Nitrate poisoning occurs through metabolism in the liver and conversion of nitrate to nitrite, which acts as an intermediary.
Laminins are high-molecular weight proteins in extracellular matrix, regarded as one of the major components of the basement membrane.
Objectives: The current study aimed to evaluate the effects of nitrate on laminin 5 expression in rat renal glomeruli.
Methods: The current interventional study was conducted on 24 male Wistar rats in Iran during 2014 - 2016.
Samples were randomly divided into four groups as follows: 1) Normal drinking water, 2) Distilled water containing 45 mg/L of nitrate, 3) Distilled water containing 100 mg/L of nitrate and 4) Distilled water (control).
Sample size consisted of 48 kidneys randomly collected from rats.
Laminin 5 content in renal glomeruli was determined using the immunohistochemistry (IHC) protocol.
Data analysis was performed using the Kruskal-Wallis test and the Spearman correlation coefficient; P value below 0.05 was considered statistically significant.
Results: In the study, intensity of reaction to anti-laminin was not the same in renal glomeruli.
While there was no significant difference between the groups one and two (P > 0.05), a significant difference was observed between the groups three and four (P < 0.05) in this regard.
Conclusions: According to the results of the study, standard amount of nitrate in drinking water had no significant impact on laminin content.
On the other hand, exceeded nitrate concentrations limited the distribution of laminin, leading to potential adverse effects on glomerular basement membrane.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jalali, Mahdi& Nikravesh, Muhammad Rida& Dorafshani, Mahmud Moghaddam. 2016. Immunohistochemical evaluation of the effects of nitrate in drinking water on laminin alpha-5 expression in rat renal glomeruli. Iranian Red Crescent Medical Journal،Vol. 18, no. 12, pp.1-7.
https://search.emarefa.net/detail/BIM-758450
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jalali, Mahdi…[et al.]. Immunohistochemical evaluation of the effects of nitrate in drinking water on laminin alpha-5 expression in rat renal glomeruli. Iranian Red Crescent Medical Journal Vol. 18, no. 12 (Dec. 2016), pp.1-7.
https://search.emarefa.net/detail/BIM-758450
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jalali, Mahdi& Nikravesh, Muhammad Rida& Dorafshani, Mahmud Moghaddam. Immunohistochemical evaluation of the effects of nitrate in drinking water on laminin alpha-5 expression in rat renal glomeruli. Iranian Red Crescent Medical Journal. 2016. Vol. 18, no. 12, pp.1-7.
https://search.emarefa.net/detail/BIM-758450
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 6-7
رقم السجل
BIM-758450
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر