PAX5a and PAX5b mRNA expression in breast cancer : relation to serum P53 and MMP2

الملخص EN

Background: Many studies evaluated the role of paired box gene 5 (PAX5) in breast cancer.

However, few investigated PAX5a and PAX5b isoforms individually.

Objective: The aim of the present study is to evaluate mRNA expression of PAX5a and PAX5b in breast cancer and assessing their underlying pathological roles through investigating their correlations to matrix metalloproteinase2 (MMP2), p53 and cancer antigen 15-3 (CA15-3).

Methods: Evaluation of PAX5a and PAX5b RNA expression in breast tissue samples was done by real-time PCR, serum MMP2 and p53 by ELISA and CA15-3 by chemiluminescent immunoassay in 55 infiltrating ductal carcinoma patients, 25 fibroadenoma patients and 15 control females who underwent plastic breast surgery.

Results: PAX5a RNA was reduced while PAX5b RNA was elevated in breast cancer patients compared to other groups (p < 0.01).

The best cutoff value of PAX5a was 61.15 with 78.2% sensitivity and 90% specificity and for PAX5b it was P2.01 with 83.6% sensitivity and 82.5% specificity.

The RNA expression of PAX5b was higher in advanced grades and stages (p < 0.01), and significantly correlated to p53 (r: 0.51, p < 0.01), and CA15-3 (r: 0.45, p < 0.01).

Moreover, PAX5a was negatively correlated to PAX5b (r: 42, p < 0.01), p53 (r: 53, p < 0.01) and MMP2 (r:).

Conclusions: The downregulation of PAX5a with upregulation of PAX5b RNA expressions could highlight the possible oncogenic role of PAX5 as a transcriptional factor in tumorigenesis and progression of breast cancer.