Effects of acetamiprid and glyph sate pesticides on testis and serum testosterone level in male mice

الملخص EN

This research is carried out to evaluate the potential toxicity of the acetamiprid and glyphosate pesticides individually and in combination through studying histology of testis, diameters and thicknesses of seminiferous tubules and serum testosterone levels in male albino mice.

The study was conducted on thirty five male mice distributed randomly into seven groups.

The first group served as control group, received tap water.

The second and the third groups received 0.16 ml/L and 0.22 ml/L of acetamiprid, respectively.

The fourth group received 3.125 ml/L of glyphosate, and the fifth group received 4.166 mg/L of glyphosate as well.

Group six received combination of equal volumes of 0.16 ml/L acetamiprid and 3.125 ml/L glyphosate.

Group seven received a combination of equal volumes of 0.22 ml/L acetamiprid and 4.166 ml/L glyphosate.

The pesticides were applied in mice drinking water for a period of four weeks.

The separate tested doses of acetamiprid and glyphosate pesticides induced alterations in serum testosterone levels.

The alterations that were caused by acetamiprid were in expected manner, the adverse effects were increased by increasing the exposure doses.

While for glyphosate the results were different by the fact that the second dose of glyphosate caused significant increase in comparison with the first one.

Histological examinations showed that the alterations in architecture of seminiferous tubules, their diameters and thicknesses, caused by both pesticides separately, were increased by increasing the exposure doses.

Regard the combinations of the two pesticides, histological sections revealed severe changes in both combinations especially in group seven, while serum testosterone showed that the first combination did not cause significant changes in comparison with both pesticides, and the second combination caused significant changes in comparison with acetamiprid alone.

It can be concluded from this study that acetamiprid and glyphoste are responsible for the alterations in serum testosterone, diameters and thicknesses of the seminiferous tubules, and combinations of both pesticides have synergistic effects on testis histology in male albino mice.