Phenotypic and genotypic identification of flouroquinolones resistant escherichia coli isolated from diseased chicken in Suez Canal District
العناوين الأخرى
التعريف الظاهري و الجيني للميكروب القولوني المقاوم للفلوروكينولون المعزول من الدجاج المصاب في منطقة قناة السويس
المؤلفون المشاركون
al-Shadhili, Daliyah Abd al-Rahman
Nasif, Suad Abd al-Aziz Abd al-Wanis
Mahmud, Fadwa Fathi
المصدر
Kafr El-Sheikh Veterinary Medical Journal
العدد
المجلد 12، العدد 1 (30 يونيو/حزيران 2014)، ص ص. 1-22، 22ص.
الناشر
جامعة كفر الشيخ كلية الطب البيطري
تاريخ النشر
2014-06-30
دولة النشر
مصر
عدد الصفحات
22
التخصصات الرئيسية
الملخص EN
A total of 70 Escherichia coli strains were isolated from samples (liver and heart) collected from diseased chickens (n=105) in about 21 commercial farms located in Suez Canal district of Egypt during May-July 2012.
We investigated the susceptibility to Flouroquinolones (Ciprofloxacin, Enrofloxacin and Norfloxacin) and 9 other antibacterials (Gentamicin, Streptomycin, Doxycyclin, Amoxicillin, Ampicillin, Cephazolin, Cephradine, Lincomycin, Florfenicole) by disc diffusion method and measured the MIC of ciprofloxacin and enrofloxacin.
Resulted flouroquinolones resistant E.coli isolates were then serotyped, and investigated for the prevalence of plasmidmediated fluoroquinolone resistance (PMQR) genes (qnrA, qnrB, qnrS, aac(6`)-Ib-cr, and qepA) by polymerase chain reaction.
Among the E.coli isolates, 72.8% (51/70) were resistant to Ciprofloxacin and Enrofloxacin with MIC ≥ 4 and ≥ 2 (μg/ml) respectively.
These isolates were resistant also to at least 6 other investigated antibacterials in the study for susceptibility/resistance.
Among the resistant 51 E.coli strains, 36 (70.5%) were positive for PMQR genes, 27 (52.9%) were positive for at least one gene of the qnr genes investigated in the study, 16 (31.37%) were positive for qnrA, qnrB and qnrS separately, 20 (39.2%) were positive to aac(6`)-Ib-cr, 35 (68.6%) were positive to qepA, while 4 (7.8%) harbored all the 5 investigated genes.
The resistance of E.
coli to flouroquinolones has been increasing in the past twenty years but unfortunately no enough data about isolates harboring PMQR genes in Egypt either in human or poultry field.
Further investigations concerning the antimicrobial resistance problem in human and veterinary field in Egypt is recommended as a surveillance program is a must.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Shadhili, Daliyah Abd al-Rahman& Nasif, Suad Abd al-Aziz Abd al-Wanis& Mahmud, Fadwa Fathi. 2014. Phenotypic and genotypic identification of flouroquinolones resistant escherichia coli isolated from diseased chicken in Suez Canal District. Kafr El-Sheikh Veterinary Medical Journal،Vol. 12, no. 1, pp.1-22.
https://search.emarefa.net/detail/BIM-761326
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Shadhili, Daliyah Abd al-Rahman…[et al.]. Phenotypic and genotypic identification of flouroquinolones resistant escherichia coli isolated from diseased chicken in Suez Canal District. Kafr El-Sheikh Veterinary Medical Journal Vol. 12, no. 1 (2014), pp.1-22.
https://search.emarefa.net/detail/BIM-761326
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Shadhili, Daliyah Abd al-Rahman& Nasif, Suad Abd al-Aziz Abd al-Wanis& Mahmud, Fadwa Fathi. Phenotypic and genotypic identification of flouroquinolones resistant escherichia coli isolated from diseased chicken in Suez Canal District. Kafr El-Sheikh Veterinary Medical Journal. 2014. Vol. 12, no. 1, pp.1-22.
https://search.emarefa.net/detail/BIM-761326
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 15-22
رقم السجل
BIM-761326
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر