Isolation and identification of bacteria from burn injuries by 16S rRNA gene

الملخص EN

Burn injuries are common and major health problem throughout the world.

The burn wound represents as a favorable area for opportunistic colonization of microorganisms with exogenous and endogenous origin.

In burns patients infections arise from multiple sources.

Burn wounds become initially colonized bacteria, mainly Staphylococci, that are superseded during the second week by Gram negative bacteria.

this study was aimed Isolation and identification of bacteria from burn injuries by 16S rRNA Gene .

Materials A total of 107 wound samples Who admitted to referral of Hussein Teaching Hospital of the period from 2016 to 2015.

All the samples were cultured on medias MacConkey agar , Blood agar, Nutrient agar and mannitol salt agar for the isolation of Gram negative bacteria and Gram positive bacteria.

After confirmation through colony characteristics and biochemical tests, API20 and Viteck2,as well as to molecular dignostics using 16S rRNA gene.

all the isolates were evaluated against commonly used antibiotics, for their susceptibility using Viteck system.

As for the screening test sensitivity was shown the results of the current study that the most effective antibiotics against both Gram-negative bacilli bacteria and Gram- positive cocci were Ceftazidin, Ciprofloxacin, Amicacin while most of isolates showed high resistance to Ticarkcillin, Gentamicin, Impenen.