Proteomic analysis of differentially expressed proteins induced by salicylic acid in suspension-cultured ginseng cells
المؤلفون المشاركون
Sun, Jiaman
Zhou, Rujun
Fu, Junfan
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 21، العدد 2 (30 إبريل/نيسان 2014)، ص ص. 185-190، 6ص.
الناشر
تاريخ النشر
2014-04-30
دولة النشر
السعودية
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
In this study, optimized 2-DE sample preparation methodologies were established for suspension-cultured ginseng cells.
Three commonly used protein extraction methods (Trichloroacetic acid-acetone, urea/thiourea and phenol extraction method) were evaluated for proteomic analysis of suspension cultures of ginseng.
A comparative analysis of suspension-cultured ginseng cells proteome induced by salicylic acid (SA) was reported.
The results demonstrated that phenol extraction method was the best method based on protein extraction efficiency and the good quality of 2-DE patterns for suspension-cultured ginseng cells.
Fifteen differentially expressed proteins induced by salicylic acid in suspension-cultured ginseng cells were identified by MALDI-TOFMS.
These identified proteins were involved in defense and stress response, energy metabolism, signal transduction/transcription, protein synthesis and metabolism, and photosynthesis.
Chaperonin 60, related to defense responses, was more abundant in suspension-cultured ginseng cells after application of SA.
Vacuolar ATPase subunit B was newly induced in SA treatment
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sun, Jiaman& Fu, Junfan& Zhou, Rujun. 2014. Proteomic analysis of differentially expressed proteins induced by salicylic acid in suspension-cultured ginseng cells. Saudi Journal of Biological Sciences،Vol. 21, no. 2, pp.185-190.
https://search.emarefa.net/detail/BIM-766860
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sun, Jiaman…[et al.]. Proteomic analysis of differentially expressed proteins induced by salicylic acid in suspension-cultured ginseng cells. Saudi Journal of Biological Sciences Vol. 21, no. 2 (Apr. 2014), pp.185-190.
https://search.emarefa.net/detail/BIM-766860
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sun, Jiaman& Fu, Junfan& Zhou, Rujun. Proteomic analysis of differentially expressed proteins induced by salicylic acid in suspension-cultured ginseng cells. Saudi Journal of Biological Sciences. 2014. Vol. 21, no. 2, pp.185-190.
https://search.emarefa.net/detail/BIM-766860
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 189-190
رقم السجل
BIM-766860
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر