Investigate the different effect of nicotine on H460 and H441 lung cells viability
المؤلفون المشاركون
Muhammad, Bushra Jasim
al-Thuwayni, Aminah Nimah
المصدر
al-Kindy College Medical Journal
العدد
المجلد 13، العدد 1 (30 يونيو/حزيران 2017)، ص ص. 92-96، 5ص.
الناشر
تاريخ النشر
2017-06-30
دولة النشر
العراق
عدد الصفحات
5
التخصصات الرئيسية
الموضوعات
الملخص EN
Background : Nicotine is the foremost chemical constituent responsible for addiction in tobacco products, in the non-ionized condition can be easily absorbed via epithelial tissue of the lung, the mouth, the nose and across the skin Objective:The study examines the harmful effect of the nicotine which is an important component of cigarette in vitro.
Type of the study: Cross-sectional study.
Methods: Examines the harmful effect of the nicotine which is an important component of cigarette in vitro by using two types of lung cancer cell lines (H460 TP53+/+, H441 TP53-/-).
Results: The results showed the total count of H460( TP53+/+) cancer lung cell lines was (5.2 ×106 cells /ml) , the number (4.9 ×106 cells /ml) of them were alive and (3.6×105 cells /ml) of them were dead, with percentage of viability (93.15%), while the total count of H441( TP53-/-) lung cancer cells was (5.1 ×106 cells /ml), the number (4.1 ×106 cells /ml) of them were alive, and the number (9.9×105 cells /ml) of them were dead with percentage of viability (80.55%).
And it revealed that the nicotine inhibited viability of H460 lung cancer cell lines in all concentrations (1, 10, 500 and 1000 μ M) and the cells were completely abolished by treatment with (1000 μ M) when the viability reached to minimum percentage (3.43%), while nicotine induced proliferation in H441 lung cancer cell lines even in lowest concentration (1 μ M), whereas the viability reached to maximum percentage (41.04%) at concentration (1000 μ M).
Also it noticed that the treatment with nicotine at concentrations (1,10, 500 and 1000 μ M ) for 24 and 48 hr induced the apoptosis but not necrosis in H460 lung cancer cell lines, especially at the highest concentrations (1000 μ M) for 48hr when the percentage of apoptosis reached to the maximum value (55.5%), however it was induced proliferation in H441 lung cancer cell lines with highest proliferation at concentration (1000 μ M), when the percentage apoptosis value reached to the minimum percentage (1.5%) when compared to un treated cells (control).
Conclusions: the cells lack to TP53(H441 TP53-/-) will proliferate when exposed to nicotine and some of these cells will suffer from necrosis as a replacement of apoptosis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Muhammad, Bushra Jasim& al-Thuwayni, Aminah Nimah. 2017. Investigate the different effect of nicotine on H460 and H441 lung cells viability. al-Kindy College Medical Journal،Vol. 13, no. 1, pp.92-96.
https://search.emarefa.net/detail/BIM-767879
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Muhammad, Bushra Jasim& al-Thuwayni, Aminah Nimah. Investigate the different effect of nicotine on H460 and H441 lung cells viability. al-Kindy College Medical Journal Vol. 13, no. 1 (2017), pp.92-96.
https://search.emarefa.net/detail/BIM-767879
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Muhammad, Bushra Jasim& al-Thuwayni, Aminah Nimah. Investigate the different effect of nicotine on H460 and H441 lung cells viability. al-Kindy College Medical Journal. 2017. Vol. 13, no. 1, pp.92-96.
https://search.emarefa.net/detail/BIM-767879
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 95-96
رقم السجل
BIM-767879
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر