Virulence genes profile of Pseudomonas aeruginosa local isolates from burns and wounds
المؤلفون المشاركون
al-Shama, Nur Fuad Kazim
al-Fahham, Muhammad Abd al-Ikhwah
Abu Rishah, Rasmiyah Abd
المصدر
Iraqi Journal of Biotechnology
العدد
المجلد 15، العدد 3 (31 ديسمبر/كانون الأول 2016)، ص ص. 31-39، 9ص.
الناشر
جامعة بغداد معهد الهندسة الوراثية و التقنيات الإحيائية للدراسات العليا
تاريخ النشر
2016-12-31
دولة النشر
العراق
عدد الصفحات
9
التخصصات الرئيسية
الموضوعات
الملخص EN
In this study a total of 111 swabs were collected from 50 patients suffering from burns and wounds between the age 9 months -69 years from both gender , the specimens were collected from Educational Al-Yarmouk and AL Kadhimiya hospitals in Baghdad during the period between October-December\2014, All the samples were identified by biochemical tests , API 20 E and Vitak -2 .
From 111 specimens, 31 isolates were Pseudomonas aeruginosa , 33 isolates were Escherichia coli and 10 isolates were Klebsiella spp, .
Drug susceptibility tests for the 31 isolates of Pseudomonas aeruginosa were studied by the disk diffusion method against 5 antibiotics and the result showed the percentage of resistance: Carbenicillin 100%, Ticarcillin 61 %, Pipracillin 32 % ,Cefprozil 93% , Colistin 84% .
The DNA samples of the 31 isolates of Pseudomonas.aeruginosa were extracted by DNA extraction kit , the concentration for all 31 DNA samples were between 60- 110 ng /ul and the purity were between 1.8-2 .PCR (Polymerase Chain Reactin) was used for screening the virulence factor genes , protein synthesis inhibition genes (exo A , las B , exoU ) of the thirty one isolates of Pseudomonas aeruginosa .
The result showed that 27 ( 87%) isolates were positive for exo A and las B genes, 4 ( 13%) were negative, , from 25 burn isolates was 23 (92%) positive isolates and from 6 wound isolates was 4 (66%) positives isolates.
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For the exoU gene the result showed that 17 ( 55%) were positive , 14 ( 45% )were negative , From 25 burn isolates the presence of exoU was seen in 15 ( 60 %), while from 6 wound isolates was 2 (33 %).
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Shama, Nur Fuad Kazim& Abu Rishah, Rasmiyah Abd& al-Fahham, Muhammad Abd al-Ikhwah. 2016. Virulence genes profile of Pseudomonas aeruginosa local isolates from burns and wounds. Iraqi Journal of Biotechnology،Vol. 15, no. 3, pp.31-39.
https://search.emarefa.net/detail/BIM-769533
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Shama, Nur Fuad Kazim…[et al.]. Virulence genes profile of Pseudomonas aeruginosa local isolates from burns and wounds. Iraqi Journal of Biotechnology Vol. 15, no. 3 (2016), pp.31-39.
https://search.emarefa.net/detail/BIM-769533
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Shama, Nur Fuad Kazim& Abu Rishah, Rasmiyah Abd& al-Fahham, Muhammad Abd al-Ikhwah. Virulence genes profile of Pseudomonas aeruginosa local isolates from burns and wounds. Iraqi Journal of Biotechnology. 2016. Vol. 15, no. 3, pp.31-39.
https://search.emarefa.net/detail/BIM-769533
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 38-39
رقم السجل
BIM-769533
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر