Production, partial purification and antitumor properties of bioactive compounds from locally isolated actinomycetes (KH14)
المؤلفون المشاركون
Risan, Muhsin Hashim
Muhammad Amin, Samman M.
Abd al-Muhaymin, Nidal
المصدر
Iraqi Journal of Biotechnology
العدد
المجلد 15، العدد 3 (31 ديسمبر/كانون الأول 2016)، ص ص. 51-64، 14ص.
الناشر
جامعة بغداد معهد الهندسة الوراثية و التقنيات الإحيائية للدراسات العليا
تاريخ النشر
2016-12-31
دولة النشر
العراق
عدد الصفحات
14
التخصصات الرئيسية
الموضوعات
الملخص EN
The present study was conducted to evaluate the antimicrobial and antitumor activities of bioactive compounds produced from locally isolated actinomycetes (KH14), and analyzing these compounds by TLC, bioautograhgy and HPLC-MS.
The isolate KH14 showed significant inhibition of human microbial pathogens, both Gram positive, Gram negative bacteria and yeast with an inhibition zone of 30, 13 and 12 mm, respectively.
The MICs potential against Staphylococcus aureus, Escherichia coli and Candida albicans were 64, 500 and 500 μl/ml, respectively.
The antimicrobial compound was analyzed by TLC, which indicated the presence of four spots while only one of them exhibited antimicrobial activities and their position was determined by bioautography, via different solvents exhibiting different Rf values.
HPLS-MS analysis of scraped active spot from the TLC indicated the presence of a large number of compounds when determining their mass chromatogram, while UV absorbance indicated the presences of only two peaks with UV absorbances 221.85and 264.85nm.
The antitumor activity by 3-(4, 5-dimethylthiazol- 2-yl) -2, 5-diphenyl-tetrazolium bromide (MTT) assay indicated that KH14possesses a powerful effect against using cancerous cell lines, especially against Breast cancer cells (MCF-7) by inhibiting 75.84% with (400 μg), while against the rest of other cell lines, Human Prostate cancer cells ( PC3)and Human malignant melanoma, skin cancer (A375), were shown moderate activities.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Risan, Muhsin Hashim& Abd al-Muhaymin, Nidal& Muhammad Amin, Samman M.. 2016. Production, partial purification and antitumor properties of bioactive compounds from locally isolated actinomycetes (KH14). Iraqi Journal of Biotechnology،Vol. 15, no. 3, pp.51-64.
https://search.emarefa.net/detail/BIM-769534
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Risan, Muhsin Hashim…[et al.]. Production, partial purification and antitumor properties of bioactive compounds from locally isolated actinomycetes (KH14). Iraqi Journal of Biotechnology Vol. 15, no. 3 (2016), pp.51-64.
https://search.emarefa.net/detail/BIM-769534
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Risan, Muhsin Hashim& Abd al-Muhaymin, Nidal& Muhammad Amin, Samman M.. Production, partial purification and antitumor properties of bioactive compounds from locally isolated actinomycetes (KH14). Iraqi Journal of Biotechnology. 2016. Vol. 15, no. 3, pp.51-64.
https://search.emarefa.net/detail/BIM-769534
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 63-64
رقم السجل
BIM-769534
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر