Efficacy of using gama interferon assay for evaluation of vaccination with sheep pox vaccine against lumpy skin disease in cattle
العناوين الأخرى
كفاء استخدام قياس الانترفيرون جاما فى ا فى تقيم التحصين بلقاح جدري الأغنام ضد مرض الجلد العقدي في الماشية
المؤلفون المشاركون
Basyuni, Ayat Allah Ibrahim
Mikhail, Christine Aziz
Abd al-Rahman, Walid
المصدر
Suez Canal Veterinary Medicine Journal
العدد
المجلد 21، العدد 2 (31 ديسمبر/كانون الأول 2016)، ص ص. 109-123، 15ص.
الناشر
جامعة قناة السويس كلية الطب البيطري
تاريخ النشر
2016-12-31
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الموضوعات
الملخص EN
Critical considerations for optimizing and conducting assays for large population based studies are feasibility, amount of sample aliquots, processing time and cost.
Well-designed statistical approaches that quickly identify optimal conditions for a given assay could assist efficient completion of the laboratory assays, in the present study, the kinetics of interferon (IFN) expression was determined in whole blood after in vitro stimulation with sheep pox vaccine (SPV) to determine whether a rapid method to detect cell mediated immune responses to sheep pox (SPV) vaccination against lumpy skin disease virus (LSDV) in cattle and could be used either as a diagnostic test or to provide a correlation of protection in animals post-vaccination.
Using protocols based on the BOVIGAM assay for tuberculosis, whole blood samples from (SPV) vaccinated and control cattle, before and after vaccination, were incubated with different amounts of SPV and the supernatants were harvested at different intervals and assayed for interferon-gamma (IFN-γ) by an enzyme-linked immunosorbent assay (ELISA).
24 hours culture was suitable for estimating IFN-γ release.
Specific induction of IFN was detected in samples from vaccinated cattle after different time intervals post vaccination, however INF-γ can be detected 5 days post vaccination reaching its maximum at 14 day post vaccination on contrast to the antibody detection by serum neutrization test (SNT) and ELISA which detected at 15 days post vaccination (DPV) reaching its maximum 28 days PV.
Combining the results of the IFN-γ assay with SNT antibody titre and ELISA, provided the capacity of INF-γ assay to evaluate the immune status of vaccinated cattle with SPV against LSD as further development of this assay may provide a useful tool for the determination of LSD immune status, including the identification of vaccinated and infected cattle.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Basyuni, Ayat Allah Ibrahim& Mikhail, Christine Aziz& Abd al-Rahman, Walid. 2016. Efficacy of using gama interferon assay for evaluation of vaccination with sheep pox vaccine against lumpy skin disease in cattle. Suez Canal Veterinary Medicine Journal،Vol. 21, no. 2, pp.109-123.
https://search.emarefa.net/detail/BIM-774514
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Basyuni, Ayat Allah Ibrahim…[et al.]. Efficacy of using gama interferon assay for evaluation of vaccination with sheep pox vaccine against lumpy skin disease in cattle. Suez Canal Veterinary Medicine Journal Vol. 21, no. 2 (2016), pp.109-123.
https://search.emarefa.net/detail/BIM-774514
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Basyuni, Ayat Allah Ibrahim& Mikhail, Christine Aziz& Abd al-Rahman, Walid. Efficacy of using gama interferon assay for evaluation of vaccination with sheep pox vaccine against lumpy skin disease in cattle. Suez Canal Veterinary Medicine Journal. 2016. Vol. 21, no. 2, pp.109-123.
https://search.emarefa.net/detail/BIM-774514
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 120-122
رقم السجل
BIM-774514
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر