Efficient and reproducible in vitro regeneration of Solanum lycopersicum and assessment genetic uniformity using flow cytometry and SPAR methods

المؤلفون المشاركون

al-Attar, Abd al-Rahman A.
Abd al-Salam, Islam M.
Canto, Tomas
Saquib, Quaiser
Javed, Sad B.
al-Khudayri, Abd al-Aziz A.
al-Shaykh, Muhammad Abd al-Rauf
Faysal, Muhammad

المصدر

Saudi Journal of Biological Sciences

العدد

المجلد 24، العدد 6 (30 سبتمبر/أيلول 2017)، ص ص. 1430-1436، 7ص.

الناشر

الجمعية السعودية لعلوم الحياة

تاريخ النشر

2017-09-30

دولة النشر

السعودية

عدد الصفحات

7

التخصصات الرئيسية

الطب البشري

الملخص EN

In the present study, we develop an efficient and reproducible in vitro regeneration system for two cultivars viz., Jamila and Tomaland of Solanum lycopersicum L., an economically important vegetable crop throughout the world.

Sterilization of seeds with 2.5 % (v/v) NaOCl was found to be most effective, about 97% of seeds germinated on cotton in magenta box moistened with sterile half strength (½)Murashige and Skoog (MS) medium.

Regeneration efficiency of cotyledonary leaf (CL) and cotyledonary node (CN) explants derived from 08 days old aseptic seedling were assessed on MS medium supplemented with different concentrations of auxins and cytokinin.

CL explants were found more responsive in comparison to CN in both the cultivars.

Types of basal media were also assessed and found to have a significant effect on shoot regeneration.

Highest regeneration frequency and maximum number of shoots were standardized from CL explants on MS medium supplied with 6-benzyl adenine (BA; 5.0 mM), indole-3-butyric acid (IBA; 2.5 mM) and Kinetin (Kin; 10.0 mM).

In vitro regenerated microshoots were rooted on ½MS medium containing 0.5 mMindole-3-butyric acid (IBA).

Regenerated plantlets with well-developed roots and shoot system were successfully acclimated to ex vitro condition.

Genetic uniformity of tissue culture raised plantlets was first time evaluated using flow cytometry and single primer amplification reaction (SPAR) methods viz., DAMD and ISSR.

No significant changes in ploidy level and nuclear DNA content profile were observed between in vitro propagated plants and normal plants of both the cultivars.

Similarly, the SPAR analysis also revealed monomorphic banding patterns in regenerated plantlets of S.

lycopersicum verifying their genetic uniformity and clonal fidelity.

This efficient regeneration system can be used as a fast and reproducible method for genetic transformation of this important vegetable crop.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

al-Attar, Abd al-Rahman A.& Faysal, Muhammad& Abd al-Salam, Islam M.& Canto, Tomas& Saquib, Quaiser& al-Shaykh, Muhammad Abd al-Rauf…[et al.]. 2017. Efficient and reproducible in vitro regeneration of Solanum lycopersicum and assessment genetic uniformity using flow cytometry and SPAR methods. Saudi Journal of Biological Sciences،Vol. 24, no. 6, pp.1430-1436.
https://search.emarefa.net/detail/BIM-776466

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

al-Shaykh, Muhammad Abd al-Rauf…[et al.]. Efficient and reproducible in vitro regeneration of Solanum lycopersicum and assessment genetic uniformity using flow cytometry and SPAR methods. Saudi Journal of Biological Sciences Vol. 24, no. 6 (Sep. 2017), pp.1430-1436.
https://search.emarefa.net/detail/BIM-776466

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

al-Attar, Abd al-Rahman A.& Faysal, Muhammad& Abd al-Salam, Islam M.& Canto, Tomas& Saquib, Quaiser& al-Shaykh, Muhammad Abd al-Rauf…[et al.]. Efficient and reproducible in vitro regeneration of Solanum lycopersicum and assessment genetic uniformity using flow cytometry and SPAR methods. Saudi Journal of Biological Sciences. 2017. Vol. 24, no. 6, pp.1430-1436.
https://search.emarefa.net/detail/BIM-776466

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references : p. 1436

رقم السجل

BIM-776466