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Culture-dependent bacteria in commercial fishes : qualitative assessment and molecular identification using 16S rRNA gene sequencing
المؤلفون المشاركون
Alikunhi, Nabil M.
Batang, Zenon B.
al-Jahdali, Haytham A.
Aziz, Muhammad A. M.
al-Suwaylim, Abd al-Aziz M.
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 24، العدد 6 (30 سبتمبر/أيلول 2017)، ص ص. 1105-1116، 12ص.
الناشر
تاريخ النشر
2017-09-30
دولة النشر
السعودية
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Fish contamination has been extensively investigated along the Saudi coasts, but studies pertaining to bacterial pathogens are scarce.
We conducted qualitative assessment and molecular identification of culture-dependent bacteria in 13 fish species from three coastal sites and a local fish market in Jeddah, Saudi Arabia.
Bacterial counts of gills, skin, gut and muscle were examined on agar plates of Macconkey’s (Mac), Eosin Methylene Blue (EMB) and Thiosulfate Citrate Bile Salts (TCBS) culture media.
Bacterial counts significantly differed between species, sources and feeding habits of examined fishes.
Mugil cephalus exhibited higher counts on TCBS (all body parts), Mac (gills, muscle and gut) and EMB (gills and muscle).
Fishes from Area I had higher bacterial loads, coinciding with those in seawater and sediment from the same site, indicating direct association between habitat conditions and the levels of bacterial contamination.
By feeding habit, detritivorous fish harbored higher counts than herbivorous and carnivorous species.
Bacterial counts of skin were higher in fish from market than field sites, and positively correlated with other body parts indicating the relation of surface bacterial load on the overall quality of fish.
Rahnella aquatilis (Enterobacteriaceae) and Photobacterium damselae (Vibrionaceae) were among the dominant species from fish muscle based on 16S rRNA sequencing.
These species are known human pathogens capable of causing foodborne illness with severe antibiotic resistance.
Opportunistic pathogens, e.g.
Hafnia sp.
(Enterobacteriaceae) and Pseudomonas stutzeri (Pseudomonadaceae) also occurred in fish muscle.
The inclusion of bacterial contamination in future monitoring efforts is thus crucial.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Alikunhi, Nabil M.& Batang, Zenon B.& al-Jahdali, Haytham A.& Aziz, Muhammad A. M.& al-Suwaylim, Abd al-Aziz M.. 2017. Culture-dependent bacteria in commercial fishes : qualitative assessment and molecular identification using 16S rRNA gene sequencing. Saudi Journal of Biological Sciences،Vol. 24, no. 6, pp.1105-1116.
https://search.emarefa.net/detail/BIM-776493
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Alikunhi, Nabil M.…[et al.]. Culture-dependent bacteria in commercial fishes : qualitative assessment and molecular identification using 16S rRNA gene sequencing. Saudi Journal of Biological Sciences Vol. 24, no. 6 (Sep. 2017), pp.1105-1116.
https://search.emarefa.net/detail/BIM-776493
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Alikunhi, Nabil M.& Batang, Zenon B.& al-Jahdali, Haytham A.& Aziz, Muhammad A. M.& al-Suwaylim, Abd al-Aziz M.. Culture-dependent bacteria in commercial fishes : qualitative assessment and molecular identification using 16S rRNA gene sequencing. Saudi Journal of Biological Sciences. 2017. Vol. 24, no. 6, pp.1105-1116.
https://search.emarefa.net/detail/BIM-776493
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 1115-1116
رقم السجل
BIM-776493
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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