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Vitrification assessment of buffalo (Bubalus bubalis) oocytes : morphological and molecular aspects
المؤلفون المشاركون
al-Sayyid, Ashraf Abd al-Halim
Jad, Ahmad Yahya
Khalifah, Muhammad Hasan
Hasan, Jamal Ashur
المصدر
Egyptian Journal of Animal Production
العدد
المجلد 52، العدد (sup) (30 إبريل/نيسان 2015)، ص ص. 1-7، 7ص.
الناشر
الجمعية المصرية للإنتاج الحيواني
تاريخ النشر
2015-04-30
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Successful cryopreservation of oocytes would preserve the genetic material from superior animals.
However, oocyte cryopreservation is relatively less successful comparing to embryos and spermatozoa due to the extreme sensitivity to chilling.
Therefore, the objective of this study was to determine the effect of vitrification process before or after oocyte maturation on the expression level of three quality related genes and on the nuclear and cytoplasmic maturation rates of vitrified immature buffalo oocytes.
Oocytes were collected and vitrified either immediately after collection as immature oocyte then thawed and matured in vitro (IV group) or vitrified after maturation (MV group).
Fresh collected and matured oocytes were used as control group.
Maturation rate was determined by cumulus cells expansion (cytoplasmic maturation) and by presence of first polar body (nuclear maturation).
The mRNA expression analysis was performed to assess the expression of three quality related genes (SOD1, BAX and MAPK14).
The results demonstrated that cryopreservation of immature oocytes did not affect cytoplasmic maturation but significantly reduced the extrusion of the first polar body to 10.6 % compared to 34.1% of control group.
BAX transcript showed no significant differences among all groups, although it was down-regulated in IV group and up-regulated in MV ones.
However, SOD1 was significantly (P< 0.05) down-regulated in MV group and up-regulated in IV group compared to control group.
MAPK14 was significantly (P< 0.05) down-regulated in IV group.
In conclusion, this study indicated the deleterious effect of vitrification process on nuclear maturation and expression level of the selected genes in buffalo oocytes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Sayyid, Ashraf Abd al-Halim& Hasan, Jamal Ashur& Khalifah, Muhammad Hasan& Jad, Ahmad Yahya. 2015. Vitrification assessment of buffalo (Bubalus bubalis) oocytes : morphological and molecular aspects. Egyptian Journal of Animal Production،Vol. 52, no. (sup), pp.1-7.
https://search.emarefa.net/detail/BIM-777424
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Sayyid, Ashraf Abd al-Halim…[et al.]. Vitrification assessment of buffalo (Bubalus bubalis) oocytes : morphological and molecular aspects. Egyptian Journal of Animal Production Vol. 52, no. (sup) (Apr. 2015), pp.1-7.
https://search.emarefa.net/detail/BIM-777424
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Sayyid, Ashraf Abd al-Halim& Hasan, Jamal Ashur& Khalifah, Muhammad Hasan& Jad, Ahmad Yahya. Vitrification assessment of buffalo (Bubalus bubalis) oocytes : morphological and molecular aspects. Egyptian Journal of Animal Production. 2015. Vol. 52, no. (sup), pp.1-7.
https://search.emarefa.net/detail/BIM-777424
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 5-6
رقم السجل
BIM-777424
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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