Effects of glutathione and the aqueous extract of Nigella sativa seeds on liver, kidney and testis tissues damaged by hydrogen peroxide in male mice

الملخص EN

Objective : This study was designed to investigate the effect of glutathione and the aqueous extract of Nigella sativa seeds extraction liver, kidney and testis tissues of male mice have been treated with H2O2.

Methods: The mice were divided into four groups (each group contain eight male mice), the first group (control) injected with 0.1ml (normal saline) /daily for 30 days, the second group injected with 0.1 ml H2O2 (0.5%) / daily for 20 day, the third group injected with 0.1 ml H2O2 (5%) /daily for 20 days and treated by Glutathione 100 mg/kg/ daily/ for 10days, the fourth group injected with 0.1 ml H2O2 (0.5%) daily for 20 days and treated with 100 mg/kg from the extract of Nigella Sativa seeds for 10days.

Results: The results showed a significant decrease (p≤0.05) in liver enzymes (AST, ALT and ALP) in all groups compared with control group and a significant increase in the third and fourth group compared with the second group.

As the results showed a significant increase (p≤0.05) in urea and Creatinine concentration in the second group compared with control group.

The result of histological study showed inflammation , hypertrophy of nuclei hepatocytes, degenerated of hepatocytes increase Kupffer cell and bloody congestion in liver of mice and there are hemorrhage, fibrosis, apoptosis and hyperplasia in kidney of the second group.

Furthermore, the results revealed a significant decrease (p≤0.05) in Spermatogonia in all groups compared with control group and a significant decrease in primary spermatocytes and spermatids in the second group compared with other groups.

As the results showed a significant decrease(p≤0.05) in the sperm count in all groups compare with the control group and a significant increase in the third and fourth group compared with the second group.

While there were a significant increase in rate of sperm abnormalities in the second group compared with the other groups.

Conclusion: The Results indicated that there were negative effects of hydrogen peroxide in male mice and the treatment of mice with glutathione and aqueous extract of Nigella sativa seeds reduced those effects at different rates.