Citrinin detection by intensified fluorescence signal of a FRET-based immunosensor using magnetic silica core-shell
المؤلفون المشاركون
Bayat, Mansur
Tajik, Parviz
Sadi, Behrooz Shojaee
Hashimi, Sayyid Jamal
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 25، العدد 1 (31 يناير/كانون الثاني 2018)، ص ص. 171-177، 7ص.
الناشر
تاريخ النشر
2018-01-31
دولة النشر
السعودية
عدد الصفحات
7
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الملخص EN
The specific immune-reaction between the anti-citrinin antibody immobilized on the surface of magnetic/silica core–shell (MSCS) and the citrinin–Rho123–BSA conjugate brings the Rho123 fluorophore as an acceptor and the QDs as a donor in close spatial proximity and causes FRET for occurring upon photo-excitation of the QDs.
The novelties of this study include : (1) immobilization of the MSCS; (2) large amount of the immobilized QDs, and (3) immobilization of a large amount of Rho123 on the BSA macromolecule.
Cd/Te QDs were synthesized by the simultaneous reduction of cadmium chloride and tellurium in the presence of sodium borohydride.
Magnetic nanoparticles were synthesized using FeSO4 and FeCl3.
The prepared magnetic nanoparticles shelled by silica using tetraethoxysilane in the presence of ammonia.
Transmission electron microscopy (TEM) analysis was used for investigating shape and monodispersity of the nanoparticles.
EDC / NHS was used as a cross linking agent for immobilization of the QDs, conjugation of citrinin to amino groups of BSA, labeling of BSA with Rho123 and also for immobilization of the amino-functionalized MSCS on the immobilized QDs.
Immobilization of the anti-citrinin antibody on the surface of the amino-functionalized MSCS was performed by Schiff-base mechanism.
By using these three effective strategies, sensitivity of the designed nanobiosensor was incredibly enhanced as a very low limit of detection (up to 0.1 pM).
The feasibility of this technique was tested by the detection of citrinin in the spiked human serum.
Results showed that there was a linear correlation between the decreased fluorescence intensity of the Rho123 and increased fluorescence intensity of the QDs with increasing concentration of citrinin in the spiked samples in the range of 1–6 pM.
According to obtained results, we conclude that this highly sensitive detection scheme is a easy, quick and impressive method that can be used in optical-based nanosensors.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sadi, Behrooz Shojaee& Bayat, Mansur& Tajik, Parviz& Hashimi, Sayyid Jamal. 2018. Citrinin detection by intensified fluorescence signal of a FRET-based immunosensor using magnetic silica core-shell. Saudi Journal of Biological Sciences،Vol. 25, no. 1, pp.171-177.
https://search.emarefa.net/detail/BIM-780411
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sadi, Behrooz Shojaee…[et al.]. Citrinin detection by intensified fluorescence signal of a FRET-based immunosensor using magnetic silica core-shell. Saudi Journal of Biological Sciences Vol. 25, no. 1 (Jan. 2018), pp.171-177.
https://search.emarefa.net/detail/BIM-780411
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sadi, Behrooz Shojaee& Bayat, Mansur& Tajik, Parviz& Hashimi, Sayyid Jamal. Citrinin detection by intensified fluorescence signal of a FRET-based immunosensor using magnetic silica core-shell. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 1, pp.171-177.
https://search.emarefa.net/detail/BIM-780411
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 176-177
رقم السجل
BIM-780411
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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