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A new diagnostic tool for rapid and accurate detection of mycobacterium tuberculosis
المؤلفون المشاركون
Ibrahim Zadah, Nayereh
Siadat, Sayyid Davar
Vaziri, Farzam
Bahrmand, Ahmad Rida
Nur Nimat Allah, Ali
Yari, Shamsi
Tasbiti, Ali Rida Hadi Zadah
Fathi, Abd al-Fadl
Ghazanfari, Murtada
Abd al-Rahimi, Farid
Pourazar, Shahin
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 25، العدد 3 (31 مارس/آذار 2018)، ص ص. 418-425، 8ص.
الناشر
تاريخ النشر
2018-03-31
دولة النشر
السعودية
عدد الصفحات
8
التخصصات الرئيسية
الموضوعات
الملخص EN
Mycobacterium tuberculosis, acid fast bacilli from the family of Mycobacteriaceae, is the causative agent of most cases of tuberculosis.
Tuberculosis, as a communicable disease, remains a serious public health threat, killing more than one million people globally every year.
Primary diagnosis of tuberculosis bacilli (TB) relies mainly on microscopic detection of acid fast bacilli (AFB), but the method suffers from low sensitivity and the results largely depend on the technician’s skill.
New diagnostic tools are necessary to be introduced for rapid and accurate detection of the bacilli in sputum samples.
We, in collaboration with Anda Biologicals, have developed a new platform, named as ‘‘Patho-tb”, for rapid detection of AFB with high sensitivity and with low dependence on human skills.
Evaluation of Patho-tb test performance was done in two settings : (1) primary field study conducted using 38 sputa from high TB prevalence area of Iran (Zabol city near to the Afghanistan border), and (2) main study conducted using 476 sputa from Tehran, capital of Iran.
Patho-tb was applied for processed sputum samples in parallel with routine diagnostic methods( including AFB microscopy, culture and PCR).
All test results were compared to final clinical diagnostic state of an individual and diagnostic sensitivity (DSe), specificity, positive predictive value, negative predictive value and accuracy of each test results were calculated using standard formulations.
Analytical sensitivity and specificity of the Patho-tb test were also determined.
Calculated values for five above mentioned parameters are as follows : for field study : (AFB DSe : 29.6 DSp : 81.8, PPV : 80, NPV : 23.1, AC : 44.7), Patho-tb (DS e: 63, DSp : 72.7, PPV : 85, NPV :44,4 AC : 65.8), and for main study : AFB (DSe : 86.1, DSp : 99.4, PPV : 98.5, NPV : 93.9, AC: 95.2), Patho-tb (DSe : 97.4, DSp : 92.9, PPV : 86.5, NPV : 98.7, AC : 94.3).
Reproducibility of Patho-tb test results were near to 100 % (Cohen’s kappa value between 0.85 and 1).
The detection limit of Patho-tb test with 100 % positivity rate was 3×103 cells / ml of sputum.
In the field study, Patho-tb test was 33.4 % more sensitive than AFB microscopy, while the improvement was only 11.3 % during the main study.
Patho-tb results are easy to interpret and the test can be merged with other screening tests, like AFB.
Totally, Patho-tb test alone or in conjunction with AFB microscopy is a useful screening tool for TB detection especially in poor geographical lab conditions.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nur Nimat Allah, Ali& Siadat, Sayyid Davar& Yari, Shamsi& Tasbiti, Ali Rida Hadi Zadah& Ibrahim Zadah, Nayereh& Vaziri, Farzam…[et al.]. 2018. A new diagnostic tool for rapid and accurate detection of mycobacterium tuberculosis. Saudi Journal of Biological Sciences،Vol. 25, no. 3, pp.418-425.
https://search.emarefa.net/detail/BIM-780669
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Nur Nimat Allah, Ali…[et al.]. A new diagnostic tool for rapid and accurate detection of mycobacterium tuberculosis. Saudi Journal of Biological Sciences Vol. 25, no. 3 (Mar. 2018), pp.418-425.
https://search.emarefa.net/detail/BIM-780669
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nur Nimat Allah, Ali& Siadat, Sayyid Davar& Yari, Shamsi& Tasbiti, Ali Rida Hadi Zadah& Ibrahim Zadah, Nayereh& Vaziri, Farzam…[et al.]. A new diagnostic tool for rapid and accurate detection of mycobacterium tuberculosis. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 3, pp.418-425.
https://search.emarefa.net/detail/BIM-780669
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 424-425
رقم السجل
BIM-780669
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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