Expression of variable viruses as herpes simplex glycoprotein D and varicella zoster gE glycoprotein using a novel plasmid based expression system in insect cell

المؤلفون المشاركون

al-Harbi, Khalid K.
al-Sulayman, A. M.
Vallely, P. J.
Klapper, P. E.
al-Baradii, Raid
al-Mabruk, Shaihana Abd al-Rahman

المصدر

Saudi Journal of Biological Sciences

العدد

المجلد 24، العدد 7 (30 نوفمبر/تشرين الثاني 2017)، ص ص. 1497-1504، 8ص.

الناشر

الجمعية السعودية لعلوم الحياة

تاريخ النشر

2017-11-30

دولة النشر

السعودية

عدد الصفحات

8

التخصصات الرئيسية

الأحياء
الطب البشري

الموضوعات

الملخص EN

Several prokaryotic and eukaryotic expression systems have been used for in vitro production of viruses’ proteins.

However eukaryotic expression system was always the first choice for production of proteins that undergo post-translational modification such as glycosylation.

Recombinant baculoviruses have been widely used as safe vectors to express heterologous genes in the culture of insect cells, but the manipulation involved in creating, titrating, and amplifying viral stocks make it time consuming and laborious.

Therefore, to facilitate rapid expression in insect cell, a plasmid based expression system was used to express herpes simplex type 1 glycoprotein D (HSV-1 gD) and varicella zoster glycoprotein E (VZV gE).

Recombinant plasmids were generated, transfected into insect cells (SF9), and both glycoproteins were expressed 48 h post-infection.

A protein with approximately molecular weight of 64-kDa and 98-kDa for HSV-1 gD and VZV gE respectively was expressed and confirmed by SDS.

Proteins were detected in insect cells cytoplasm and outer membrane by immunofluorescence.

The antigenicity and immunoreactivity of each protein were confirmed by immunoblot and ELISA.

Results suggest that this system can be an alternative to the traditional baculovirus expression for small scale expression system in insect cells.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

al-Sulayman, A. M.& Vallely, P. J.& Klapper, P. E.& al-Baradii, Raid& al-Mabruk, Shaihana Abd al-Rahman& al-Harbi, Khalid K.. 2017. Expression of variable viruses as herpes simplex glycoprotein D and varicella zoster gE glycoprotein using a novel plasmid based expression system in insect cell. Saudi Journal of Biological Sciences،Vol. 24, no. 7, pp.1497-1504.
https://search.emarefa.net/detail/BIM-781642

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

al-Sulayman, A. M.…[et al.]. Expression of variable viruses as herpes simplex glycoprotein D and varicella zoster gE glycoprotein using a novel plasmid based expression system in insect cell. Saudi Journal of Biological Sciences Vol. 24, no. 7 (Nov. 2017), pp.1497-1504.
https://search.emarefa.net/detail/BIM-781642

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

al-Sulayman, A. M.& Vallely, P. J.& Klapper, P. E.& al-Baradii, Raid& al-Mabruk, Shaihana Abd al-Rahman& al-Harbi, Khalid K.. Expression of variable viruses as herpes simplex glycoprotein D and varicella zoster gE glycoprotein using a novel plasmid based expression system in insect cell. Saudi Journal of Biological Sciences. 2017. Vol. 24, no. 7, pp.1497-1504.
https://search.emarefa.net/detail/BIM-781642

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references : p. 1503-1504

رقم السجل

BIM-781642