Molecular diagnosis of patulin toxic produced from penicillium expansum in apples fruits

المؤلفون المشاركون

al-Husayni, Ibtihal Muizz Abd al-Mahdi
Kazim, Khadijah Hamzah

المصدر

Mesopotamia Environment Journal

الناشر

جامعة بابل مركز البحوث و الدراسات البيئية

تاريخ النشر

2017-12-31

دولة النشر

العراق

عدد الصفحات

10

التخصصات الرئيسية

العلوم الطبيعية والحياتية (متداخلة التخصصات)

الملخص الإنجليزي

The present study aimed to test the ability of P.expansum to producing patulin toxic in some varieties of apples imported which founded in the local markets of the province of Babylon, and studying the molecular chara cteristics of toxic by using PCR technique and TLC.

Results showed that P.expansum was the highest appearance in the red apples imported, which amounted to 80 % and has the highest frequency in red apples imported (68.75) % and the lowest frequency in the apple golden yellow imported (17.3) % .

There are four isolates of the P.expansum was ability to produce patulin ( B, D, E and F) as the production rate of patulin 90 % of the isolates were studied by TLC method, as well as the detection capability isolates fungus to produce patulin by Polymerase chain reaction (PCR) using primers (P450, IDHI, G3PDH, MSAS) .The technic of PCR was proved the presence of the gene( IDH) responsible for the production of patulin in four isolates of the P.expansum of the total 13 isolates where it was noted that the gene site has appeared at the base of nitrogen 500 bp also proved the existence of a gene MSAS in 2 isolates at the base of nitrogen 300 bp.

As for the G3PDH gene it has been obtained 4 isolates a carrier of the gene, while not prove possession of the fungus isolates the gene P450.

It was also investigating the viability of P.expansum isolates to produced three types of enzymes which Amylase , Protease and Lipase.

نوع البيانات

أوراق مؤتمرات

رقم السجل

BIM-787085

• APA
• MLA
• AMA