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Auxin-cytokinin synergism in vitro for producing genetically stable plants of ruta graveolens using shoot tip meristems
المؤلفون المشاركون
al-Attar, Abd al-Rahman A.
Ahmad, Nasim
Qahtan, Ahmad A.
Anis, Muhammad
Faysal, Muhammad
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 25، العدد 2 (28 فبراير/شباط 2018)، ص ص. 273-277، 5ص.
الناشر
تاريخ النشر
2018-02-28
دولة النشر
السعودية
عدد الصفحات
5
التخصصات الرئيسية
الموضوعات
الملخص EN
An efficient micropropagation protocol was developed for Ruta graveolens Linn.
using shoot tip meristems derived from a 4-month-old field grown plant.
In vitro shoot regeneration and proliferation was accomplished on Murashige and Skoogs (MS) semi-solid medium in addition to different doses of cytokinins viz.6- benzyl adenine (BA), Kinetin (Kn) or 2-isopetynyl adenine (2iP), singly or in combination with auxins viz.
indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or a-naphthalene acetic acid (NAA).
Highest regeneration frequency (27.6 %) was obtained on (MS) medium composed of BA (10 mM) with maximum number (9.4) of shoots and 4.3 cm shoot length after 4 weeks of incubation.
Among various combinations tried best regeneration frequency (71 %) of multiple shoot formation with highest number (12.6) of shoots per shoot tip explants were achieved in MS medium augmented with a combination BA (10.0 mM) and NAA (2.5 mM) after 4 weeks of incubation.
The optimum frequency (97 %) of rhizogenesis was achieved on half-strength MS medium having 0.5 mM IBA after 4 weeks of incubation.
Tissue culture raised plantlets with 5–7 fully opened leaves with healthy root system were successfully acclimatized off in SoilriteTM with 80% survival rate followed by transportation to normal soil under natural light.
Genetic stability among in vitro raised progeny was evaluated by ISSR and RAPD markers.
The entire banding pattern revealed from in vitro regenerated plants was monomorphic to the donor.
The present protocol provides an alternative option for commercial propagation and fruitful setting up of genetically uniform progeny for sustainable utilization and germplasm preservation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Faysal, Muhammad& Ahmad, Nasim& Anis, Muhammad& al-Attar, Abd al-Rahman A.& Qahtan, Ahmad A.. 2018. Auxin-cytokinin synergism in vitro for producing genetically stable plants of ruta graveolens using shoot tip meristems. Saudi Journal of Biological Sciences،Vol. 25, no. 2, pp.273-277.
https://search.emarefa.net/detail/BIM-787335
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Faysal, Muhammad…[et al.]. Auxin-cytokinin synergism in vitro for producing genetically stable plants of ruta graveolens using shoot tip meristems. Saudi Journal of Biological Sciences Vol. 25, no. 2 (Feb. 2018), pp.273-277.
https://search.emarefa.net/detail/BIM-787335
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Faysal, Muhammad& Ahmad, Nasim& Anis, Muhammad& al-Attar, Abd al-Rahman A.& Qahtan, Ahmad A.. Auxin-cytokinin synergism in vitro for producing genetically stable plants of ruta graveolens using shoot tip meristems. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 2, pp.273-277.
https://search.emarefa.net/detail/BIM-787335
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 277
رقم السجل
BIM-787335
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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