Acids content of senniella spongiosa (f.v muell)‎ allen

الملخص EN

Snniella spongiosa (F.v.

Muell.) Allen variety holocarpa (F.v.

Muell.) Allen belongs to family Chenopodiaceae, was collected from the selected habitat Alexandria–Mersa Mattruh road) at 30 km during the vegetative growth seasons of 2003-2004.

The chromatographic investigation of the phenolic acids content of S.

spongiosa stems and leaves revealed that the leaves contain phenolic acids, ferulic, chlorogenic, caffeic and gallic acids.

While the plant stems contain the phenolic acids 2, 3-dihydroxy benzoic acid (Oprotocatechuic acid), ferulic, chlorogenic and p-hydroxyl benzoic acid These phenolic compounds were isolated using polyamide column chromatography, purified on a sephadex LH-20 column and identified through Rf-values and colour reactions, UV spectral data, 1H-NMR, 13CNMR spectra and mass spectrum Using HPLC technique, it was observed that both plant stems and leaves contain the same organic acids (oxalic, malic, acetic, citric and fumaric) with different ranges of concentration.

Acetic acid was the highest separated organic acid detected in the plant leaves and stems Investigation of free amino acids using amino acid analyzer, showed that stems and leaves of S.

spongiosa contained 13 and 14 free amino acids, respectively. Serine and proline were the highest separated free amino acid for the stems and leaves, respectively.

Glutamic acid was the highest separated amino acid (after protein hydrolysis) for the stems and leaves, respectively. Using gas liquid chromatography showed the presence of 13 and 10 hydrocarbons in the stems and leaves, respectively with different ranges of concentrations.

N-eicosane was the highest detected hydrocarbon for the stems and leaves.

Cholesterol and β- sitosterol were detected in plant stems and leaves with different ranges of concentrations Using gas liquid chromatography revealed the presence of 7 saturated fatty acids beside 2 unsaturated fatty acids in plant stems and leaves, respectively with different ranges of concentrations.

Myristic acid was the highest separated fatty acid for both plant stems and leaves.