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Detection of genetic variants in HLA-DQA1 gene with thyrotoxicosis patients in Thi-Qar City
المؤلفون المشاركون
Maghtuf, Majidah G.
al-Yasiri, Muhammad Hashim
Jabbar, Inas Abd al-Karim
Salih, Manal Badi
المصدر
al-Kufa University Journal for Biology
العدد
المجلد 9، العدد 3 (31 ديسمبر/كانون الأول 2017)، ص ص. 31-45، 15ص.
الناشر
جامعة الكوفة كلية العلوم قسم علوم الحياة
تاريخ النشر
2017-12-31
دولة النشر
العراق
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
This study was conducted to describe the single nucleotide polymorphism (SNPs) within the exon 2 of HLA-DQA1, a gene that encodes a MHC class II antigenthat localized in the surface of many immune cells and they play a central role in the immune system by presenting peptides derived from extracellular proteins, as well asto analyze the consequences of non-synonymous SNPs in this gene using several in silicobioinformatics tools in the patients with Hyper thyrotoxicosis Blood samples were collected, DNA was extracted, and one pair of specific PCR primers was designed to amplify exon 2 of thisgene.
PCR experiments were optimized and performed,and PCR products were purified and sequenced from both termini.
Sequencing results were analyzed, and each observed nsSNP was further analyzed by several computational tools.
Out of six observed SNPs, five of them were non-synonymous.
It was found that the deleterious effect of these non-synonymous SNPs wasvaried, and the most damaging mutation was V68F followed by K92N.
While the T49S and I99M have a mild parallel effect.However, no clear association was revealed between the observed SNPs of HLA-DQA1 gene and the progression of the Hyper thyrotoxicosis disease.
Therefore, the associative pattern of this genetic variation with Hyper thyrotoxicosis disease should be interpreted with caution.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Maghtuf, Majidah G.& Salih, Manal Badi& Jabbar, Inas Abd al-Karim& al-Yasiri, Muhammad Hashim. 2017. Detection of genetic variants in HLA-DQA1 gene with thyrotoxicosis patients in Thi-Qar City. al-Kufa University Journal for Biology،Vol. 9, no. 3, pp.31-45.
https://search.emarefa.net/detail/BIM-795483
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Maghtuf, Majidah G.…[et al.]. Detection of genetic variants in HLA-DQA1 gene with thyrotoxicosis patients in Thi-Qar City. al-Kufa University Journal for Biology Vol. 9, no. 3 (2017), pp.31-45.
https://search.emarefa.net/detail/BIM-795483
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Maghtuf, Majidah G.& Salih, Manal Badi& Jabbar, Inas Abd al-Karim& al-Yasiri, Muhammad Hashim. Detection of genetic variants in HLA-DQA1 gene with thyrotoxicosis patients in Thi-Qar City. al-Kufa University Journal for Biology. 2017. Vol. 9, no. 3, pp.31-45.
https://search.emarefa.net/detail/BIM-795483
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes appendices : p. 39-45
رقم السجل
BIM-795483
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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