Effect of plant growth regulators and medium constituents on in vitro propagation of grape (Vitis vinifera L.)‎ cv. “Black Matrouh”

الملخص EN

This study was carried out during the period 2011- 2013 at the laboratory of Tissue Culture in the National Gene Bank and Genetic Resources, Egypt; in order to obtain an efficient method for micropropagation of grape (Vitis vinifera L.) cv.

“Black Matrouh”.

The explants were cultured on full, three-quarter or half strength Murashige and Skoog (MS) medium supplemented with 6-benzylamino purine (BAP) at 0.2, 0.5, 1.0 or 1.5 mgl-1 for establishment stage.

For multiplication stage, full or three-quarter strength MS medium supplemented with BAP at 0.50, 0.75 or 1.00 mgl-1 alone or in combination with indol-3-butyric acid (IBA) at 0.10, 0.30 or 0.50 mgl-1 were tested.

Rooting stage was investigated on half strength MS medium supplemented with IBA or naphthalene acetic acid (NAA) at 0.1, 0.3, 0.5 or 1.0 mgl-1.

During the present study, grape shoot tip and nodal explants showed the highest survival percentages on three-quarter strength MS medium with 0.5 mgl-1 BAP (100% and 88.89%, respectively).

The highest number of shoots/shoot tip (1.86) was noticed on full strength MS medium with 0.5 mgl-1 BAP, while full strength MS medium with 1.0 mgl-1 BAP produced the highest shoot number/nodal cutting (1.67).

Established explants cultured on three-quarter strength MS medium with (0.75 BAP+0.50 IBA) mgl-1 or (1.00 BAP+0.30 IBA) mgl-1 produced the highest shoot number/explant (3.67 and 3.52 shoots/explant, respectively), while established explants cultured on growth regulator-free full strength MS medium showed the highest shoot length (4.94 cm).

In general, the rooting percentage was the highest on half strength MS medium with auxin compared to those on auxin-free medium.

Half strength MS medium with 0.5 mgl-1 NAA exhibited the highest roots number/shoot explant (5.50 roots/shoot) with adequate root length (4.11 cm) without significant difference compared to the longest roots (5.06 cm) which were observed on half strength MS medium with 0.5 mgl-1 IBA.