Toll-like receptor 4 gene polymorphisms in patients with urinary tract infection
المؤلفون المشاركون
al-Hilali, Hammadi A.
Salman, Ali N.
Dakhil, Ahmad H.
المصدر
العدد
المجلد 10، العدد 3 (30 سبتمبر/أيلول 2015)، ص ص. 78-89، 12ص.
الناشر
جامعة ذي قار قسم البحث و التطوير
تاريخ النشر
2015-09-30
دولة النشر
العراق
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Background: Urinary tract infection (UTIs) are considered to be the most common infections in humans.
Aims:The present study aimed to investigate the association between single nucleotide polymorphisms (SNPs) in the toll-like receptor 4 (Tlr4) gene (Thr399Ile) and the incidence of Urinary Tract Infection.
Subjects and Methods:A total of 49 patients with Urinary Tract Infection and 25 apparently healthy control were enrolled in this study.
Urine samples from patients with UTI was collected in AL-Imam AL-Hussain teaching hospital in Thi- Qar province, during the period from February 2014 to March 2015..
Urinary isolates were identified by conventional methods.DNA was extracted from the blood samples taken from these participants.
TLR4 gene was amplified with polymerase chain reaction (PCR) using specific primers.
Genotyping of the SNPs of interest was done by restriction fragment length polymorphism (RFLP).
Results:The present study was showed 26 (53.06%)isolates Escherichia coli; 9 (18.37%) isolates had Pseudomonas aeruginusa;7 (14.29 %) isolates had Klebsiella pneumonia; 4 (8.16%) had Proteus and 3 (6.12%) isolates had Klebsiellaoxytoca, the results showed the presence of heterozygous in one sample from study group at site 399 (C / T) after using restriction enzyme Hinf1.
Conclusion: Escherichia coli was most common causative agent in UTI, Thr399Ile may be considered as a risk factor that increases susceptibility to Urinary TractInfection
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Hilali, Hammadi A.& Salman, Ali N.& Dakhil, Ahmad H.. 2015. Toll-like receptor 4 gene polymorphisms in patients with urinary tract infection. University of Thi-Qar Journal،Vol. 10, no. 3, pp.78-89.
https://search.emarefa.net/detail/BIM-808243
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Hilali, Hammadi A.…[et al.]. Toll-like receptor 4 gene polymorphisms in patients with urinary tract infection. University of Thi-Qar Journal Vol. 10, no. 3 (Sep. 2015), pp.78-89.
https://search.emarefa.net/detail/BIM-808243
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Hilali, Hammadi A.& Salman, Ali N.& Dakhil, Ahmad H.. Toll-like receptor 4 gene polymorphisms in patients with urinary tract infection. University of Thi-Qar Journal. 2015. Vol. 10, no. 3, pp.78-89.
https://search.emarefa.net/detail/BIM-808243
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 86-89
رقم السجل
BIM-808243
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر