Evaluation of sperm apoptosis in Iraqi severe oligozoospermic patients using annexin-V by flow cytometry
المؤلفون المشاركون
Hasan, Ghassan Muhammad
Abd al-Hasan, Ismail A.
المصدر
Iraqi Journal of Biotechnology
العدد
المجلد 16، العدد 3 (30 سبتمبر/أيلول 2017)، ص ص. 186-193، 8ص.
الناشر
جامعة بغداد معهد الهندسة الوراثية و التقنيات الإحيائية للدراسات العليا
تاريخ النشر
2017-09-30
دولة النشر
العراق
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The présent study w'as conductcd in the institute of gcnctic engineering and biotcchnology - university of Baghdad during a period from 15 Scptcmber 2015 to 10 Fcbruary 2016.
The aim of this study was to dctcct the apoptotic altérations in sperm of Iraqi patients with sevcrc oligozoospermia depending on annexin V- binding using flow cytomctry and study its rclationship with some semen parameters.
Scmen samplcs w'crc taken from severc oligozoospermie (SOS) patients (n=75) and apparcntly hcalthy (AH) subjccts (fertile, n=25) by masturbation following 3-7 days of sexual abstinences in Baghdad spccialist fertility Centre.
Semcn samplcs were evaluated according to the WHO critcria which includc volume, count and the percentages of motility, active, normal and abnormal sperm.
Sperm apoptosis was determined using annexin V-FITC kit (Aviscera Biosciencc, USA) by flow cytomctry.
The percentages of livc sperms were significantly (p<0.01) higher in AH subjccts than in SOS patients.
The percentages of early and latc apoptotic sperms were significantly (p<0.01) lowcr in AH subjccts than in SOS patients.
Apoptotic index values were significantly (p<0.01) lowcr in AH subjccts than in SOS patients.
Semcn volume significantly (p<0.01) corrclatcd with the perccntage of viable sperm (r = 0.76) and invcrsely corrclatcd (p<0.01) with the percentages of early and latc apoptosis and apoptotic index.
Sperm count was invcrsely corrclatcd (p<0.01) with the percentages of early and latc apoptosis and apoptotic index, sperm motility percentages were invcrsely corrclatcd (p<0.01) with the percentages of early and latc apoptosis and apoptotic index.
The percentages of both active and normal sperm were invcrsely corrclatcd (p<0.01) with the percentages of early, latc apoptotic and apoptotic index.
In conclusion, our results showed that the percentages of apoptotic and nccrotic spermatozoa dctcctcd by flow cytomctry may be used as biomarkers for the détection of fertility.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hasan, Ghassan Muhammad& Abd al-Hasan, Ismail A.. 2017. Evaluation of sperm apoptosis in Iraqi severe oligozoospermic patients using annexin-V by flow cytometry. Iraqi Journal of Biotechnology،Vol. 16, no. 3, pp.186-193.
https://search.emarefa.net/detail/BIM-811045
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hasan, Ghassan Muhammad& Abd al-Hasan, Ismail A.. Evaluation of sperm apoptosis in Iraqi severe oligozoospermic patients using annexin-V by flow cytometry. Iraqi Journal of Biotechnology Vol. 16, no. 3 (2017), pp.186-193.
https://search.emarefa.net/detail/BIM-811045
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hasan, Ghassan Muhammad& Abd al-Hasan, Ismail A.. Evaluation of sperm apoptosis in Iraqi severe oligozoospermic patients using annexin-V by flow cytometry. Iraqi Journal of Biotechnology. 2017. Vol. 16, no. 3, pp.186-193.
https://search.emarefa.net/detail/BIM-811045
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 192-193
رقم السجل
BIM-811045
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر