تقييم التأثيرات الجزيئية للسماق على الكبد و الكلى لذكور الفئران البيض السويسرية

الملخص EN

The aim of this study was to evaluate the effect of the water extract on the DNA of the liver and kidney of male Swiss white rats by using the polymorphic randomization technique of the RAPD.

Thirty groups of mice were divided into six groups for each group of five mice.

The first group was the control group and was injected with water.

The second group, the third, fourth, fifth, and sixth groups were activated with concentrations of 6.25,20,30,50,100 mg / kg respectively Eleven days.

The animals were anesthetized and then explained to obtain liver and kidney samples to extract DNA from them, which was amplified using the RAPD technique with twelve decimal bases.

The results showed that exposure to the sumac at different concentrations affected the DNA of the liver and kidney cells of the mice and caused significant changes in the patterns of RAPD technique compared with the control group in terms of the difference in the number, size and intensity of the beams.

Therefore, it can be said that the use of high concentrations of sumac can affect DNA, which may lead to harmful mutations, and RAPD is very useful in detecting the effect of chemicals at the molecular level.