Biosynthesis of silver nanoparticles and evaluate its activity in promoting burns healing in rabbits

الملخص EN

This study was conducted to synthesis silver nanoparticles by using olive leaves extract and evaluate its activity in promoting burns healing in rabbits.

The synthesis and characterization of silver nanoparticles was confirmed by Ultra Violet Visible- spectrophotometer and Scanning Electron Microscopy.

For this purpose, twenty five rabbits were used and divided randomly into equal five groups and subjected to second degree burn under general anesthesia by bar with brass circular end of 2.5cm diameter which immersed in boiling water (100°C) for 10 min and immediately it was placed on the rabbit back for 15 sec.

without exerting any pressure just the weight of the instrument which was 350gm., these groups were: 1- Burns without any treatment and kept as negative control.

2- Burns treated with silver nanoparticles 3- Burns infected with P.

aeruginosa and don’t receive any treatment.

4-Burns infected with P.

aeruginosa and treated with silver sulfadiazine and kept as positive control.

5-Burns infected with P.

aeruginosa and treated with silver nanoparticles.

Burn healing was assessed by measuring burn diameter at 7, 14, 21 and 28 days post burn treatment.

The results of this study showed it possible to produce silver nanoparticles in ecofriendly and easy process and the results of burn healing demonstrated superiority of silver nanoparticles in reducing burn diameter at the end of the experiment.

It could be concluded that olive leaves extract can be used effectively in the production of silver nanoparticles and these synthesized nanoparticles had positive effect in enhance burn healing when its applied locally