Effect of vitamin E plus Selenium against lead exposure on histopathology of kidney and liver in common carp Cyprinus carpio L

الملخص EN

The present study was undertaken to investigate the effects of lead (Pb) either alone or with Vitamin E (VE) plus Selenium (Se) on histopathological changes in Cyprinus carpio.

For this purpose, 120 common carp with an average weight 65.50±0.25 g, and total length 17.4±2.5 cm were randomly divided into 6 treatment groups in duplicate.

Each of the six treatment groups was fed the formulated diets for 10 weeks at a daily rate of 3% body weight throughout the experiment as the following: Treatment 1 (T1) serve as control group; Treatment 2 (T2) were fed diet mixed with VE + Se (300 mg kg-1 d.w); Treatment 3 (T3) were fed diet mixed with Pb (20 mg kg-1 d.w; Treatment 4 (T4) were fed diet mixed with Pb (30 mg kg-1 d.w); Treatment 5 (T5) were fed diet mixed with Pb (20 mg kg-1 d.w) with VE+Se; Treatment 6 (T6) were fed diet mixed with Pb (30 mg kg-1 d.w) with VE+Se.

After 10 weeks feeding trail 3 fish tank-1 (6 fish treatment-1) were dissected out liver and kidney were removed and fixed in 10% formaldehyde solution for histopathological studies.

The liver of control group exhibited a quite normal architecture, while the fish exposed to Pb showed vacuolation and necrosis.

These hepatic alterations were more evident in fish exposed to (30mg kg-1 dw).

The kidney exhibited Bowman space dilatation, acute cellular degeneration and necrosis.

The quantitative histopathology revealed abnormalities in kidney and liver in all dietary Pb treated groups alone and with VE+Se.

The severity of lesion in both organs were significantly different (P<0.05) in Pb groups in comparison to Pb with VE+Se groups.

These results indicated that histopathology could be used as a tool for assessing the risk of contaminants in the environment