Effect of probiotics on some aspects of quail's health

الملخص EN

The study was carried out to explained the effect of probiotic (protexin® and lactobacillus acidophilus), Saccharomyces cerevisiae (prebiotic) and immunmodulator (levamisole) in feed additive and in drinking water on immune response post vaccination and challenge with Newcastle disease virus.

Three hundred and fifty quails were divided into five groups contained 50 chicks and as follows: Group 1: Received Saccharomyces cerevisiae in drinking water from day 1 to the day 45 in dose (107 CFU/ml).

Group 2: Received Levamisol with dose (10 µg/kg b.w.) in drinking water from 1 day to the 45 days.

Group 3: Received protxin® (Probiotic) in feed from 1 day to the 45 days in dose (0.5 g /kg feed).

Group 4: Received lactobacilli isolated from quail in drinking water from day 1-45 of the experiment in dose (107 CFU/ml).

Group5: Received lactobacilli isolated from quail in drinking water from day 1-3 of the experiment in dose (107 CFU/ml).

Group 6: Control positive vaccinated not treated.

Group6: Control negative not vaccinated and not treated.

At age 10 and 20 day All quail groups were vaccinated except the control negative with NDV vaccine La Sota strain (Intervet-Holland).

At age 42 day (sexual maturity), start collect the eggs products for hatching.

ELISA in day 4 of the offspring will carry on estimating maternal immunity for ND.

Isolate bacteria from yolk sac of the first chick offspring.

Blood samples all groups, blood samples will be collected at day 4 to check the maternal Immunity for NDV and at day (20 and 30, 45, 75, 80 and 85) to check NDV titer by ELISA.

Swabs will be taken from cloacae at (20 and 40) days in all groups to check Salmonella and E.

coli.

Challenge with NDV will take place on day 75.

The obtained results referred Ability to use the lactic acid bacteria isolate as probiotics and its metabolites as prebiotics in quail chicks.

High growth rates parameters in quail chicks.

Significant enhancement the immune response against ND post vaccination and post challenge with field virulent isolate of NDV.

Efficacy in improving the intestinal microbial balance.

Challenge with 100LD50 NDV (106) at day 75 caused high morbidity and no mortality in all vaccinated group but high mortality and morbidity in control negative.

(protexin®, sacchromyces cervisicea, Lactobacillus acidophilus and levamisole) did not protect the chicks from infection with virulent NDV but they reduced the severity of infection