PCR detection of rmpA gene among klebsiella isolated from wound and burn infections

الملخص EN

One hundred and twenty five Gram-negative-lactose fermented bacteria grown on MacConkey agar were collected from burn and, wound infections, in Al- sadar Medical City in Al- Najaf province.

The bacterial isolates were identified according to cultural characteristics ,the results revealed that 40(32%) isolates were identified as Klebsiella spp, all of them 40(100%) isolates were belong to K.

pneumonia subsp.

pneumonia .The capsule of klebsiella isolates were tested .the result revealed all Klebsiella isolates40(100%) were capsulated ,the gene coding capsule in isolates gave positive results.

Genotypically were studied carefully using PCR technique , the results pointed out that 35 (87.5%) of bacterial isolated have rmpA gene coding for producing polysaccharide capsule