In vivo and in vitro antibacterial assessment of Nerium oleander aqueous leaf extract against bacterial pathogens and its effect in treatment of wounds

الملخص EN

The aim of the study was to evaluate the antibacterial activities of aqueous leaf extract Nerium oleander against five identified bacteria namely Gram positive bacteria Bacillus subtilis, Staphylococcus aureus, and Gram negative bacteria Pseudomonas aeruginosa, Escherichia coli and Proteus mirabilis and role of extract in treatment of wounds in laboratory animal.

Antibacterial activity of aqueous extracts was determined by disc diffusion and well diffusion method on Muller–Hinton agar.

Twelve laboratory animals (Rabbit) use to evaluate the role of aqueous leaf extract of N.

oleander on healing of wound and divided into four equal groups.

Control, Fucine ointment, N.

oleander 80% and N.

oleander 100 % groups.

As the inhibitory zones were more at 60% and 80% in comparison with 2.5 % , 5 % and 10%, and the widest were at 100 % against all bacteria species and show significant difference at (P<0.05) between susceptibility of bacteria species to different concentration of aqueous leaf extract of Nerium oleander in agar well diffusion exhibit a significantly higher zones of inhibitions against all bacteria spp.

in used at 100%, 80%, 60% in comparison with 2.5%, 5% and10% concentration more than in agar disc method, which did not exhibit any inhibitory zone against all bacteria spp.

at 2.5% and the narrowest inhibitory zones was against Bacillus subtilis, and Staphylococcus aureus.

at 5% and 10% Whenever, at 100% and 80% the widest inhibitory zones was against Pseudomonas aeruginosa and Proteus mirabilis and less than against Staph.

and Bacillus spp.

Hematological changes show significant difference at P<0.05 in erythrocyte indices and leukocyte count.

Whenever, effect of extract on treatment of wound show complete healing at 6-7 days compared with local antibiotic fucine ointment without complication.

In conclusion; Aqueous leaf extracts of N.

oleander have inhibitory activity against both gram-positive and gram-negative bacteria this indicates of the presence antimicrobial compounds.

Leaf extracts of N.

oleander at 80-100 % can be used as local antibacterial and treatment of open wounds.