Assessment of genetic variability among some rabbit breeds using random amplified polymorphic DNA technique (RAPD)
المؤلفون المشاركون
al-Shawaf, I. I. S.
Rifat, M. H.
al-Zari, M. F.
Badr, Umniyah A.
Khalil, M. H.
المصدر
Annals of Agricultural Science, Moshtohor
العدد
المجلد 53، العدد 1 (31 مارس/آذار 2015)، ص ص. 89-97، 9ص.
الناشر
تاريخ النشر
2015-03-31
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Random Amplified Polymorphic DNA (RAPD) marker was employed to assess the genetic variation and phylogenetic relationship among four rabbit breeds viz.
New Zealand White, Gabali, Baladi Red and Baladi Black reared in Egypt.
Initially, a total of 14 random primers of arbitrary sequence were used but 10 of them generated reproducible, scoreable and polymorphic bands.
Out of 131 bands scored using these primers, 74(56.48%) were recognized as Polymorphic and 57 (43.52%) as monomorphic bands.
The highest percentage of polymorphic bands was recognized for primers OPB-02 (94%) and OPB-07 (92%).
While, the lowest percentage of polymorphic bands was recognized for primers OPA-02(16%) and OPF-12 (28%).
The band sharing frequencies (BSF) was found higher between Baladi Red -Baladi Black (0.80± 0.038 ) , followed by Gabali - Baladi Black (0.71±0.079 ), New Zealand White- Baladi Black (0.70±0.096 ), New Zealand Whit- Baladi Red (0.69±0.088 )and the least BSF was found between New Zealand Whit- Gabali (0.64±0.081 ) .
Overall, there was no significant difference (P>0.05) in BSF values between breeds.
The highest genetic distance was found between Baladi Red -Baladi Black (0.87) followed by Gabali, Baladi Red (0.86), Gabali, Baladi Black(0.82), New Zealand White, Gabali (0.80), New Zealand White,Baladi Red (0.75) and the lowest genetic distance was found between New Zealand White,Baladi Black(0.73).
One primer (OPA-20) in Gabali , two primers(OPA-02,OPB-14) in Baladi Red, three primers (OPA-02,OPB-14, OPA-20) in New Zealand White and Baladi Black were found to be specific for these breeds.
The study suggests that RAPD can be successfully utilized for detecting genetic variation among the studied rabbit breeds.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Shawaf, I. I. S.& Khalil, M. H.& Rifat, M. H.& al-Zari, M. F.& Badr, Umniyah A.. 2015. Assessment of genetic variability among some rabbit breeds using random amplified polymorphic DNA technique (RAPD). Annals of Agricultural Science, Moshtohor،Vol. 53, no. 1, pp.89-97.
https://search.emarefa.net/detail/BIM-819637
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Shawaf, I. I. S.…[et al.]. Assessment of genetic variability among some rabbit breeds using random amplified polymorphic DNA technique (RAPD). Annals of Agricultural Science, Moshtohor Vol. 53, no. 1 (2015), pp.89-97.
https://search.emarefa.net/detail/BIM-819637
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Shawaf, I. I. S.& Khalil, M. H.& Rifat, M. H.& al-Zari, M. F.& Badr, Umniyah A.. Assessment of genetic variability among some rabbit breeds using random amplified polymorphic DNA technique (RAPD). Annals of Agricultural Science, Moshtohor. 2015. Vol. 53, no. 1, pp.89-97.
https://search.emarefa.net/detail/BIM-819637
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
رقم السجل
BIM-819637
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر