Phylogenetic analysis of cpn 60 gene from locally isolated Acinetobacter baumannii

العناوين الأخرى

التحليل الوراثي لجين cpn60 من بكتريا Acinetobacter baumannii المعزولة محليا

المؤلف

Hamzah, Ala Salim

المصدر

Iraqi Journal of Science

العدد

المجلد 59، العدد 1A (31 مارس/آذار 2018)، ص ص. 9-15، 7ص.

الناشر

جامعة بغداد كلية العلوم

تاريخ النشر

2018-03-31

دولة النشر

العراق

عدد الصفحات

7

التخصصات الرئيسية

الأحياء

الملخص EN

This study was aimed to analysis phylogenetic tree of the gene cpn60 in Acinetobacter baumannii that was identified in Baghdad.

Study included collection two hundred specimens (fifty from UTI, fifty from wound infection , fifty from respiratory tract infection and fifty from otitis infections) .

In primary laboratory diagnosis and confirmed by using VITEK- 2 Compact system, twenty isolates of this bacterium were indentified (10%) from total specimens.

Extraction of geneteic material to detect target gene by amplification this target gene.

DNA sequencing of all isolates was done.

Then alignment of sequencing in NCBI and draw phylogenetic tree by use Geneious 9 software among sequence of locally isolates .

The results in phylogenetic tree of cpn60 gene in locally isolates showed 4 groups of isolates different with difference source of isolation.

Then phylogenetic tree for locally isolates and high identity global isolates in gene bank was drew and its results showed 12 locally isolates not identity with standard isolates.

So, chosen isolate (AE_29) isolate from these 12 isolates and documented in national GenBank as anew isolate under accession number (LOCUS KY818056 ) of nucleotides sequence and protein ID "ARV90994.1" .

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Hamzah, Ala Salim. 2018. Phylogenetic analysis of cpn 60 gene from locally isolated Acinetobacter baumannii. Iraqi Journal of Science،Vol. 59, no. 1A, pp.9-15.
https://search.emarefa.net/detail/BIM-831269

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Hamzah, Ala Salim. Phylogenetic analysis of cpn 60 gene from locally isolated Acinetobacter baumannii. Iraqi Journal of Science Vol. 59, no. 1A (2018), pp.9-15.
https://search.emarefa.net/detail/BIM-831269

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Hamzah, Ala Salim. Phylogenetic analysis of cpn 60 gene from locally isolated Acinetobacter baumannii. Iraqi Journal of Science. 2018. Vol. 59, no. 1A, pp.9-15.
https://search.emarefa.net/detail/BIM-831269

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references : p. 15

رقم السجل

BIM-831269