Incidence of Toxoplasma Gondii in placenta of aborted fetus

العناوين الأخرى

معدل حدوث الطفيلي TOXOPLASMA GONDII في مشيمة الأجنة المجهضة

المؤلفون المشاركون

Abd Allah, Shamall Muhammad Amin
Abd al-Rahman, Kamaran K.
Abd Allah, Sardar S.
Hasan, Luzan S.

المصدر

Duhok Medical Journal

الناشر

جامعة دهوك كلية الطب

تاريخ النشر

2012-12-31

دولة النشر

العراق

عدد الصفحات

6

التخصصات الرئيسية

الطب البشري

الملخص الإنجليزي

Toxoplasma gondii infections can cause serious complications in pregnant women, leading to miscarriage, stillbirth, and birth defects.

Diagnosis of T.

gondii by Polymerase chain reaction (PCR) in human placenta of aborted fetus targeted B1 gene has been conducted.

In this study, a total of 79 blood samples and the placentas of aborted fetus were collected from woman who had abortion, at the Maternity Hospital of Erbil city, Kurdistan region, northern of Iraq.

The blood samples were examined by using a latex agglutination test (LAT).

QIAamp DNA Mini Kit method was used for genomic DNA extraction from placenta.

Polymerase chain reaction was applied on all samples and 115 bp fragment of B1 gene amplified.

Polymerase chain reaction products were run in 2.5% agarose gel and visualized by UV light after ethidium bromide staining.

Out of 79 serum samples, 75 (95%) cases were positive for anti T.

gondii antibodies by LAT.

From 79 placenta samples, nine (11.38%) were positive by PCR.

Seroprevalence of T.

gondii infection was very high among women who had abortion and a strong relation was observed between toxoplasmosis and abortion using PCR method.

نوع البيانات

أوراق مؤتمرات

رقم السجل

BIM-832000

• APA
• MLA
• AMA